Darvishzadeh Mahani Fatemeh, Khaksari Mohammad, Iranpour Maryam, Soltani Zahra, Raji-Amirhasani Alireza, Hajializadeh Zahra
Endocrinology and Metabolism Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of pathology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Basic Med Sci. 2022 Mar;25(3):390-398. doi: 10.22038/IJBMS.2022.61512.13609.
Lifestyle and eating habits affect the health and function of the body's organs, including the kidneys. The current study was carried out to determine the effects of two types of diet programs, including time restriction (TR) and calorie restriction (CR) on the histopathological changes and apoptotic molecules during acute kidney injury (AKI) in postmenopausal rats.
In this study the female rats were divided into two groups of ovariectomized (OVX) and ovary-intact (sham), then they were placed on TR and CR diets for 8 weeks; afterward, AKI was induced by injection of glycerol. Functional indices, histopathological changes, Bax, and Bcl2 were measured before and after AKI.
After AKI, creatinine, serum urea, urinary albumin excretion, kidney tissue Bax, and Bax/Bcl2 ratio increased, while glomerular filtration rate (GFR) and kidney tissue Bcl2 decreased compared with before AKI. Histopathological indices (inflammation, cellular necrosis, cell vacuolization, tubular dilatation, intratubular cast, and congestion) also confirmed renal injury. TR and CR diets improved renal injury indices and prevented an increase in the Bax/Bcl2 ratio. However, in some indices, the effects of two diets on OVX animals were not observed. In addition, none of the diets could decrease kidney weight/body weight ratio (KW/BW). The histopathological finding also showed improvement of renal status in both groups, especially in the CR diet.
The results indicated that TR and CR diets had renoprotective effects against AKI by reducing the Bax/Bcl2 ratio and improving apoptosis. The effects of CR were more than TR.
生活方式和饮食习惯会影响包括肾脏在内的身体器官的健康和功能。本研究旨在确定两种饮食方案，即限时饮食（TR）和热量限制饮食（CR），对绝经后大鼠急性肾损伤（AKI）期间组织病理学变化和凋亡分子的影响。
在本研究中，将雌性大鼠分为去卵巢（OVX）组和卵巢完整（假手术）组，然后让它们分别采用TR和CR饮食8周；之后，通过注射甘油诱导AKI。在AKI前后测量功能指标、组织病理学变化、Bax和Bcl2。
与AKI前相比，AKI后肌酐、血清尿素、尿白蛋白排泄、肾组织Bax和Bax/Bcl2比值升高，而肾小球滤过率（GFR）和肾组织Bcl2降低。组织病理学指标（炎症、细胞坏死、细胞空泡化、肾小管扩张、管腔内管型和充血）也证实了肾损伤。TR和CR饮食改善了肾损伤指标，并防止了Bax/Bcl2比值的升高。然而，在某些指标上，未观察到两种饮食对OVX动物的影响。此外，两种饮食均未降低肾重/体重比（KW/BW）。组织病理学结果还显示两组的肾脏状况均有改善，尤其是CR饮食组。
结果表明，TR和CR饮食通过降低Bax/Bcl2比值和改善细胞凋亡对AKI具有肾脏保护作用。CR的效果优于TR。
