Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Endocrinology and Metabolism Research Center, Kerman University of Medical SciencesKerman, Kerman, Iran.
Mol Biol Rep. 2024 Jul 15;51(1):812. doi: 10.1007/s11033-024-09716-x.
Estrogen has a protective impact on acute kidney injury (AKI); moreover, reducing the daily intake of calories impedes developing diseases. The present study aimed to determine the effects of calorie restriction (CR) and time restriction (TR) diets on the expression of silent information regulator 2 homolog 1 (SIRT1), transforming growth factor beta 1 (TGF-β1), and other indicators in the presence and absence of ovaries in AKI female rats.
The female rats were divided into two groups, ovariectomized (OVX) and sham, and were placed on CR and TR diets for eight weeks; afterward, AKI was induced by injecting glycerol, and kidney injury indicators and biochemical parameters were measured before and after AKI.
After AKI, the levels of urine albumin excretion rate, urea, and creatinine in serum, and TGF-β1 increased, while creatinine clearance and SIRT1 decreased in kidney tissue. CR improved kidney indicators and caused a reduction in TGF-β1 and an increase in SIRT1 in ovary-intact rats. Moreover, CR prevented total antioxidant capacity (TAC) decrease and malondialdehyde (MDA) increase resulting from AKI. Before AKI, an increase in body weight, fasting blood sugar (FBS), low-density lipoprotein (LDL), triglyceride (TG), and total cholesterol (TC), and a decrease in high-density lipoprotein (HDL) were observed in OVX rats compared to sham rats, but CR prevented these changes. The effects of TR were similar to those of CR in all indicators except for TGF-β1, SIRT1, urea, creatinine, and albumin.
The present study indicated that CR is more effective than TR in preventing AKI, probably by increasing SIRT1 and decreasing TGF-β1 in ovary-intact animals.
雌激素对急性肾损伤（AKI）具有保护作用；此外，减少每日热量摄入会阻碍疾病的发展。本研究旨在确定热量限制（CR）和时间限制（TR）饮食在 AKI 雌性大鼠存在和不存在卵巢的情况下对沉默信息调节因子 2 同源物 1（SIRT1）、转化生长因子-β1（TGF-β1）和其他指标表达的影响。
将雌性大鼠分为去卵巢（OVX）和假手术组，并进行 8 周的 CR 和 TR 饮食；之后，通过注射甘油诱导 AKI，并在 AKI 前后测量肾脏损伤指标和生化参数。
AKI 后，血清中尿白蛋白排泄率、尿素和肌酐升高，而肾组织中 TGF-β1 减少，SIRT1 减少。CR 改善了肾脏指标，并导致卵巢完整大鼠的 TGF-β1 减少和 SIRT1 增加。此外，CR 防止 AKI 引起的总抗氧化能力（TAC）下降和丙二醛（MDA）增加。在 AKI 之前，与假手术组相比，OVX 大鼠的体重、空腹血糖（FBS）、低密度脂蛋白（LDL）、甘油三酯（TG）和总胆固醇（TC）增加，高密度脂蛋白（HDL）减少，但 CR 预防了这些变化。TR 的作用与 CR 在所有指标上相似，除了 TGF-β1、SIRT1、尿素、肌酐和白蛋白。
本研究表明，CR 在预防 AKI 方面比 TR 更有效，可能是通过增加卵巢完整动物中的 SIRT1 和减少 TGF-β1 实现的。
