Ali Tayyab, Hussain Fatma, Naeem Muhammad, Khan Ajmal, Al-Harrasi Ahmed
Clinico-Molecular Biochemistry Laboratory, Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
College of Life Science, Hebei Normal University, Shijiazhuang, China.
Front Bioeng Biotechnol. 2022 May 17;10:888177. doi: 10.3389/fbioe.2022.888177. eCollection 2022.
is one of the most commonly used medicinal plants as it exhibits several pharmacological activities such as antioxidant, antibacterial, anticancer, antidiabetic, and hemolytic. The purpose of this study was to apply the nanotechnology approach for exploring the enhanced bioactivities of freshly prepared L. nanosuspensions and the phytochemical profile of seed ethanolic extract. In this study, we performed the biochemical characterization of L. ethanolic extract through High-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), and Gas chromatography (GC), and bioactivities in terms of antioxidant, antidiabetic, antibacterial, and hemolytic activities of nanosuspension and extract were competitively studied. The results revealed that the nanosuspension of seeds showed higher total phenolic (478.63 ± 5.00 mg GAE/100 g) and total flavonoid contents (192.23 ± 1.390 mg CE/100 g) than the ethanolic seed extract. The antioxidant activity was performed using the DPPH scavenging assay, and nanosuspension showed higher potential (16.74 ± 1.88%) than the extract. The antidiabetic activity was performed using antiglycation and α-amylase inhibition assays, nanosuspension showed higher antidiabetic potential [antiglycation (58 ± 0.912%)] and [bacterial α-amylase inhibition (18.0 ± 1.3675%)], respectively. Nanosuspension showed higher biofilm inhibition activity against (66.44 ± 3.529%) than the extract (44.96 ± 2.238%) and ciprofloxacin (59.39 ± 3.013%). Hemolytic activity was performed and nanosuspension showed higher hemolytic activity than the extract as 7.8 ± 0.1% and 6.5 ± 0.3%, respectively. The study showed that nanosuspension had enhanced the bioavailability of bioactive plant compounds as compared to the ethanolic extract. Therefore, nanosuspension of seed extract showed higher biochemical activities as compared to the ethanolic extract. This nanotechnology approach can be used as a platform for the development of combination protocols for the characterization of liquid state nanosuspensions in an adequate manner and also for therapeutic applications.
它是最常用的药用植物之一，因为它具有多种药理活性，如抗氧化、抗菌、抗癌、抗糖尿病和溶血活性。本研究的目的是应用纳米技术方法来探索新制备的[植物名称]纳米混悬液增强的生物活性以及种子乙醇提取物的植物化学特征。在本研究中，我们通过高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）和气相色谱（GC）对[植物名称]乙醇提取物进行了生化表征，并竞争性地研究了纳米混悬液和提取物在抗氧化、抗糖尿病、抗菌和溶血活性方面的生物活性。结果表明，[植物名称]种子的纳米混悬液比种子乙醇提取物具有更高的总酚含量（478.63±5.00毫克没食子酸当量/100克）和总黄酮含量（192.23±1.390毫克儿茶素当量/100克）。使用DPPH清除试验进行抗氧化活性检测，纳米混悬液显示出比提取物更高的潜力（16.74±1.88%）。使用抗糖化和α-淀粉酶抑制试验进行抗糖尿病活性检测，纳米混悬液分别显示出更高的抗糖尿病潜力[抗糖化（58±0.912%）]和[细菌α-淀粉酶抑制（18.0±1.3675%）]。纳米混悬液对[细菌名称]的生物膜抑制活性（66.44±3.529%）高于提取物（44.96±2.238%）和环丙沙星（59.39±3.013%）。进行了溶血活性检测，纳米混悬液的溶血活性高于提取物，分别为7.8±0.1%和6.5±0.3%。研究表明，与乙醇提取物相比，纳米混悬液提高了生物活性植物化合物的生物利用度。因此，[植物名称]种子提取物的纳米混悬液比乙醇提取物表现出更高的生化活性。这种纳米技术方法可作为一个平台，用于以适当方式开发用于表征液态纳米混悬液的组合方案，也可用于治疗应用。
