Nawaz Aqsa, Ali Tayyab, Naeem Muhammad, Hussain Fatma, Li Zhiye, Nasir Abdul
Clinico-Molecular Biochemistry Laboratory, Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
College of Life Science, Hebei Normal University, Shijiazhuang, China.
Front Chem. 2023 May 5;11:1194389. doi: 10.3389/fchem.2023.1194389. eCollection 2023.
is a traditional medicinal plant known for its anti-inflammatory, antidiabetic, antimicrobial, anticancer, and antioxidant properties. Its therapeutic efficacy using nanosuspensions is still unclear for treating infectious diseases. This study was designed to evaluate the bioactivities, biochemical characterization, and bioavailability of freshly prepared nanosuspensions of . Structural and biochemical characterization of and its biological activities, such as antioxidants, antimicrobials, antiglycation, α-amylase inhibition, and cytotoxicity was performed using Fourier-transform infrared (FTIR) spectroscopy and High-Performance Liquid Chromatography (HPLC). extract and nanosuspensions showed TPCs values of 341.88 and 39.51 mg GAE/100 g while showing TFCs as 429.19 and 239.26 mg CE/100g, respectively. DPPH inhibition potential of extract and nanosuspension was 27.3% and 10.6%, respectively. Biofilm inhibition activity revealed that bark extract and nanosuspension showed excessive growth restraint against , reaching 67.11% and 66.09%, respectively. The α-amylase inhibition assay of extract and nanosuspension was 39.3% and 6.3%, while the antiglycation activity of nanosuspension and extract was 42.14% and 53.76%, respectively. Extracts and nanosuspensions showed maximum hemolysis at 54.78% and 19.89%, respectively. Results indicated that nanosuspensions possessed antidiabetic, antimicrobial, anticancer, and antioxidant properties. Further study, however, is needed to assess the clinical studies for the therapeutic use of nanosuspensions.
是一种传统药用植物，以其抗炎、抗糖尿病、抗菌、抗癌和抗氧化特性而闻名。其纳米混悬液在治疗传染病方面的治疗效果仍不明确。本研究旨在评估新鲜制备的纳米混悬液的生物活性、生化特性和生物利用度。使用傅里叶变换红外（FTIR）光谱和高效液相色谱（HPLC）对其结构、生化特性及其生物活性（如抗氧化剂、抗菌剂、抗糖化、α-淀粉酶抑制和细胞毒性）进行了研究。提取物和纳米混悬液的总酚含量（TPCs）值分别为341.88和39.51 mg GAE/100 g，而总黄酮含量（TFCs）分别为429.19和239.26 mg CE/100g。提取物和纳米混悬液对二苯基苦味酰基自由基（DPPH）的抑制潜力分别为27.3%和10.6%。生物膜抑制活性表明，树皮提取物和纳米混悬液对[具体菌种未给出]均表现出过度的生长抑制，分别达到67.11%和66.09%。提取物和纳米混悬液的α-淀粉酶抑制率分别为39.3%和6.3%，而纳米混悬液和提取物的抗糖化活性分别为42.14%和53.76%。提取物和纳米混悬液的最大溶血率分别为54.78%和19.89%。结果表明，纳米混悬液具有抗糖尿病、抗菌、抗癌和抗氧化特性。然而，需要进一步研究以评估纳米混悬液治疗用途的临床研究。
