Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710069, China.
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an 710069, China.
ACS Appl Bio Mater. 2022 Jun 20;5(6):3067-3074. doi: 10.1021/acsabm.2c00338. Epub 2022 Jun 3.
Mn release is particularly important for biological application of manganese-based nanomaterials. However, the Mn release profiles of the manganese ferrite nanoparticles are under clarification. Here, we synthesized 3, 10, and 18 nm manganese ferrite nanoparticles (MFNPs) as model systems to study the Mn release behavior, size, and pH-dependent kinetics. The Mn release kinetic study showed that the first-order kinetic model was suitable for 3 and 10 nm MFNPs, while the Higuchi model was suitable for 18 nm MFNPs in a neutral PBS buffer (pH 7.4). In an acidic PBS buffer (pH 4.8), the Mn release from all sizes of MFNPs follows first-order kinetics, which is possible due to the reaction between MFNPs and H. The influence of Mn release was evaluated by comparing the variations of magnetic resonance (MR) relaxation and magnetic properties before and after Mn release of MFNPs. The results showed that the saturation magnetization (), longitudinal relaxivity (), and transverse relaxivity () values declined due to Mn release, while the ratio of / increased slightly, showing that all sizes of MFNPs exhibited the same MR mode as the synthesized MFNPs. More importantly, the release kinetics were employed to estimate the toxicity of the released Mn. The potential toxicity is acceptable for MFNP administration since the calculated amount of Mn is in the range of safe doses.
锰的释放对于基于锰的纳米材料的生物应用尤为重要。然而，锰铁氧体纳米颗粒的锰释放情况仍需进一步阐明。在这里，我们合成了 3、10 和 18nm 锰铁氧体纳米颗粒（MFNPs）作为模型系统，以研究锰的释放行为、尺寸和 pH 值依赖性动力学。锰释放动力学研究表明，一级动力学模型适用于 3nm 和 10nm MFNPs，而在中性 PBS 缓冲液（pH7.4）中，Higuchi 模型适用于 18nm MFNPs。在酸性 PBS 缓冲液（pH4.8）中，所有尺寸的 MFNPs 的锰释放都遵循一级动力学，这可能是由于 MFNPs 与 H 的反应所致。通过比较 MFNPs 释放前后磁共振（MR）弛豫和磁性能的变化来评估锰释放的影响。结果表明，由于锰的释放，饱和磁化强度（）、纵向弛豫率（）和横向弛豫率（）值下降，而/的比值略有增加，表明所有尺寸的 MFNPs 都表现出与合成 MFNPs 相同的 MR 模式。更重要的是，释放动力学被用来估计释放的锰的毒性。由于计算出的锰的量在安全剂量范围内，因此 MFNP 给药的潜在毒性是可以接受的。
