Singapore Institute of Food and Biotechnology Innovation, Agency for Science, Technology and Research (A⁎STAR) 1, Pesek Road, Jurong Island, 627833, Singapore.
NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore; Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore; Wilmar-NUS Corporate Laboratory (WIL@NUS), National University of Singapore, 14 Medical Drive, Singapore 117599, Singapore.
Curr Opin Biotechnol. 2022 Aug;76:102740. doi: 10.1016/j.copbio.2022.102740. Epub 2022 Jun 2.
Single-cell proteins (SCPs) have been widely used in human food and animal feed applications, still, there are challenges in their production and commercialization. Recently, advances in microbial synthetic biology, genomic engineering, and biofoundry technologies have offered capabilities to effectively and rapidly engineer microorganisms for improving the productivity, nutritional, and functional quality of SCPs. In this review, we discuss various synthetic biology, genomic engineering, and biofoundry tools that can be harnessed for SCP production and genetic modification. We also describe the current and potential applications of genetic modification in producing intermediate feedstocks, as well as biomass-based and multifunctional SCPs. Finally, we discuss the technological and policy-control related challenges encountered when deploying genetic modification in SCP production for animal feed and human food applications.
单细胞蛋白（SCP）已广泛应用于人类食品和动物饲料，但其生产和商业化仍存在挑战。最近，微生物合成生物学、基因组工程和生物制造技术的进步为有效和快速地工程改造微生物以提高 SCP 的生产力、营养价值和功能质量提供了能力。在本综述中，我们讨论了可用于 SCP 生产和遗传修饰的各种合成生物学、基因组工程和生物制造工具。我们还描述了遗传修饰在生产中间饲料、生物质基和多功能 SCP 方面的当前和潜在应用。最后，我们讨论了在 SCP 生产中部署遗传修饰用于动物饲料和人类食品应用时遇到的技术和政策控制相关挑战。
