Alimzhanova Mereke, Mamedova Madina, Ashimuly Kazhybek, Alipuly Alham, Adilbekov Yerlan
al-Farabi Kazakh National University, Faculty of Physics and Technology, 71 al-Farabi Ave., 050040 Almaty, Kazakhstan.
Center of Physical Chemical Methods of Research and Analysis, al-Farabi Kazakh National University, 050012 Almaty, Kazakhstan.
Food Chem X. 2022 May 23;14:100345. doi: 10.1016/j.fochx.2022.100345. eCollection 2022 Jun 30.
A simple and rapid method based on miniaturized solid-phase microextraction (mini-SPME) followed by gas chromatography-mass spectrometry was developed to identify eight endocrine disruptors (atrazine, diethylstilbestrol, hexestrol, estrone, estradiol, ethinylestradiol, norgestrel, and megestrel) in drinking water samples. Extraction parameters was optimized and further analyses was performed using them. The optimum temperature for the determination of endocrine disruptors in water was 80 °C; the optimum extraction and preincubation times were 60 and 20 min, respectively. The studied linear range of endocrine disruptors was 10.0-1000 μg mL. The limit of detection ranged from 0.020 to 0.087 μg mL. The correlation coefficient (r) was 0.96-0.99. This research introduces a novel method for detecting analytes at extremely low concentrations, as well as the possibility of combining several detection technologies to give high-accuracy qualitative and quantitative determination of endocrine disruptors in aqueous samples.
建立了一种基于微型固相微萃取（mini-SPME）结合气相色谱-质谱联用的简单快速方法，用于鉴定饮用水样品中的8种内分泌干扰物（阿特拉津、己烯雌酚、己烷雌酚、雌酮、雌二醇、炔雌醇、炔诺孕酮和甲地孕酮）。对萃取参数进行了优化，并使用优化后的参数进行了进一步分析。测定水中内分泌干扰物的最佳温度为80℃；最佳萃取时间和预孵育时间分别为60分钟和20分钟。所研究的内分泌干扰物线性范围为10.0-1000μg/mL。检测限为0.020-0.087μg/mL。相关系数（r）为0.96-0.99。本研究介绍了一种在极低浓度下检测分析物的新方法，以及结合多种检测技术对水样中内分泌干扰物进行高精度定性和定量测定的可能性。
