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从性别分化角度对叶片中黄酮类生物合成基因进行代谢组和转录组整合分析

Integrative Metabolome and Transcriptome Analysis of Flavonoid Biosynthesis Genes in Leaves From the Perspective of Sex Differentiation.

作者信息

Jiao Peng, Chaoyang Li, Wenhan Zhai, Jingyi Dai, Yunlin Zhao, Zhenggang Xu

机构信息

Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, Changsha, China.

Central South Inventory and Planning Institute of National Forestry and Grassland Administration, Changsha, China.

出版信息

Front Plant Sci. 2022 May 20;13:900030. doi: 10.3389/fpls.2022.900030. eCollection 2022.

DOI:10.3389/fpls.2022.900030
PMID:35668799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9163962/
Abstract

Flavonoids are important secondary metabolites involved in plant development and environmental responses. Sex differences in flavonoids are common in plants. is a dioecious plant that is rich in flavonoids. However, few studies have been done on its molecular mechanism, especially sex differences. In the present study, we performed an integrated transcriptomics and metabolomics analysis of the sex differences in the accumulation of flavonoids in leaves at different developmental stages. In general, flavonoids accumulated gradually with developmental time, and the content in female plants was higher than that in male plants. The composition of flavonoids in female and male plants was similar, and 16 kinds of flavonoids accumulated after flowering. Correspondingly, a significant enrichment of differentially expressed genes and metabolites was observed in the flavonoid biosynthesis pathway. WGCNA and qRT-PCR analyses identified several key genes regulating the accumulation of flavonoids, such as those encoding and . In addition, 8 TFs were found to regulate flavonoid biosynthesis by promoting the expression of multiple structural genes. These findings provide insight into flavonoid biosynthesis in associated molecular regulation.

摘要

黄酮类化合物是参与植物发育和环境响应的重要次生代谢产物。植物中黄酮类化合物的性别差异很常见。[植物名称]是一种富含黄酮类化合物的雌雄异株植物。然而，关于其分子机制，尤其是性别差异的研究很少。在本研究中，我们对[植物名称]不同发育阶段叶片中黄酮类化合物积累的性别差异进行了转录组学和代谢组学的综合分析。总体而言，黄酮类化合物随着发育时间逐渐积累，雌株中的含量高于雄株。雌株和雄株中黄酮类化合物的组成相似，开花后积累了16种黄酮类化合物。相应地，在黄酮类生物合成途径中观察到差异表达基因和代谢物的显著富集。WGCNA和qRT-PCR分析确定了几个调节黄酮类化合物积累的关键基因，如编码[基因名称1]和[基因名称2]的基因。此外，发现8个转录因子通过促进多个结构基因的表达来调节黄酮类生物合成。这些发现为[植物名称]中黄酮类生物合成的相关分子调控提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/9218fa45f103/fpls-13-900030-g0010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/899592f6e022/fpls-13-900030-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/9218fa45f103/fpls-13-900030-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/c897cbbc491a/fpls-13-900030-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/30a1b2a1b457/fpls-13-900030-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/d436bbdb7bfc/fpls-13-900030-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/9ba558f47845/fpls-13-900030-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/c37fb80617bb/fpls-13-900030-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/e1f4870263b3/fpls-13-900030-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/727b5069cc06/fpls-13-900030-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/d4e9a73cc2ca/fpls-13-900030-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/899592f6e022/fpls-13-900030-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/9163962/9218fa45f103/fpls-13-900030-g0010.jpg

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