利用微型计算机断层扫描研究丁香酚和槲皮素对链脲佐菌素-烟酰胺诱导的糖尿病大鼠骨质流失的影响。
Investigation of the effects of eugenol and quercetin on bone loss in STZ-NA induced diabetic rats utilizing micro CT.
作者信息
Baş Aycan, Albeniz Işıl
机构信息
Department of Biophysics, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Department of Biophysics, İstanbul Faculty of Medicine, İstanbul University, Çapa, İstanbul, Turkey.
出版信息
J Diabetes Metab Disord. 2022 Mar 12;21(1):637-646. doi: 10.1007/s40200-022-01026-y. eCollection 2022 Jun.
PURPOSE
Diabetes Mellitus (DM) is a systemic disease that can effect tissues and their physiological functions at molecular and biochemical levels. Diabetic osteoporosis is one of the chronic diseases of bone metabolism effected by and characterized by augmented risk of osteoporotic fractures and destroying of bone microarchitecture. It was aimed to investigate the alterations in femoral bone structure that may take place as a complication of DM by using the antioxidant properties of eugenol and quercetin, which are phenolic compounds, in streptozotocin nicotinamide (STZ-NA) induced rats as an experimental type 2 DM (T2 DM) model.
METHODS
The antioxidant effect of eugenol and quercetin in case of DM development was determined by GSH ELISA kit. The effect of DM on alterations in bone structure was analyzed by micro CT. BMD, Tb.Bv/Tb.Tv, Tb.N, Tb.Th, Ct.Th, Tb.Sp and SMI data were calculated with the software CTAn.
RESULTS
Serum GSH levels, Tb.Th and Tb.Bv/Tb.Tv values statistically decreased, and SMI values statistically increased in diabetic group compared with controls. Serum GSH levels in eugenol group were higher than diabetic group, and Tb.Bv/Tb.Tv values in eugenol group were lower than controls. Quercetin group had higher serum GSH levels and Tb.Th values compared with diabetic group, while SMI values were lower in quercetin group compared with diabetic group.
CONCLUSION
Eugenol and quercetin were revealed to have antioxidant, antidiabetic and osteoprotective effects on the repair of bone structure in experimental STZ-NA T2 DM model.
目的
糖尿病（DM）是一种全身性疾病，可在分子和生化水平上影响组织及其生理功能。糖尿病性骨质疏松症是受骨质疏松性骨折风险增加和骨微结构破坏影响并以其为特征的骨代谢慢性疾病之一。本研究旨在通过使用丁香酚和槲皮素（均为酚类化合物）的抗氧化特性，在链脲佐菌素烟酰胺（STZ-NA）诱导的大鼠中建立实验性2型糖尿病（T2 DM）模型，来研究作为DM并发症可能发生的股骨结构变化。
方法
通过谷胱甘肽（GSH）ELISA试剂盒测定丁香酚和槲皮素在DM发生情况下的抗氧化作用。通过显微CT分析DM对骨结构变化的影响。使用CTAn软件计算骨密度（BMD）、骨小梁骨体积分数（Tb.Bv/Tb.Tv）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）、皮质厚度（Ct.Th）、骨小梁间距（Tb.Sp）和结构模型指数（SMI）数据。
结果
与对照组相比，糖尿病组血清GSH水平、Tb.Th和Tb.Bv/Tb.Tv值统计学上降低，而SMI值统计学上升高。丁香酚组血清GSH水平高于糖尿病组，丁香酚组Tb.Bv/Tb.Tv值低于对照组。与糖尿病组相比，槲皮素组血清GSH水平和Tb.Th值更高，而槲皮素组SMI值低于糖尿病组。
结论
在实验性STZ-NA T2 DM模型中，丁香酚和槲皮素对骨结构修复具有抗氧化、抗糖尿病和骨保护作用。
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