Derakhshan Mohammad Ali, Nazeri Niloofar, Khoshnevisan Kamyar, Heshmat Ramin, Omidfar Kobra
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
J Diabetes Metab Disord. 2022 Jan 20;21(1):313-321. doi: 10.1007/s40200-022-00976-7. eCollection 2022 Jun.
Active wound dressing with physicochemical and biological characteristics is more effective in healing diabetic foot ulcer (DFU). In this study, a 3-layer electrospun nanofiber wound dressings was fabricated, while its outer, middle and inner layers of the scaffold were made of PCL, PCL/collagen and collagen nanofibers, respectively. Various amounts of Melilotus officinalis extract were also loaded in the collagen nanofibers as a biologically active compound. The diameter and morphology of the obtained nanofibers were investigated by scanning electron microscopy (SEM) and FT-IR spectroscopy to analyse the composition of prepared dressings. The efficacy of the fabricated dressings as wound healing agent was assessed in streptozotocin-induced diabetic rats. The results demonstrated that the mean diameter of nanofibers are 373 ± 179 nm, 266 ± 108 nm, 160 ± 52 nm, and 393 ± 131 nm for PCL, PCL/collagen, pure collagen, and collagen nanofibers containing 0.08 g extract, respectively. The histo-pathology and histomorphometry assessments demonstrate the herbal extract-loaded electrospun dressings (especially containing 0.08 g of the extract) are promising in improving the diabetic ulcer healing. Our results indicated that the combination of drug did not compromise the physicochemical characteristics of wound dressing, while improving its biological activities.
具有物理化学和生物学特性的活性伤口敷料在治疗糖尿病足溃疡（DFU）方面更有效。在本研究中，制备了一种三层电纺纳米纤维伤口敷料，其支架的外层、中层和内层分别由聚己内酯（PCL）、PCL/胶原蛋白和胶原蛋白纳米纤维制成。各种量的草木犀提取物也作为生物活性化合物负载在胶原蛋白纳米纤维中。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）研究所得纳米纤维的直径和形态，以分析制备的敷料的组成。在链脲佐菌素诱导的糖尿病大鼠中评估了制备的敷料作为伤口愈合剂的功效。结果表明，PCL、PCL/胶原蛋白、纯胶原蛋白和含有0.08 g提取物的胶原蛋白纳米纤维的纳米纤维平均直径分别为373±179 nm、266±108 nm、160±52 nm和393±131 nm。组织病理学和组织形态计量学评估表明，负载草药提取物的电纺敷料（特别是含有0.08 g提取物的敷料）在改善糖尿病溃疡愈合方面具有前景。我们的结果表明，药物组合在改善伤口敷料生物活性的同时，并未损害其物理化学特性。
