Faal Mina, Manouchehri Hamed, Changizi Reza, Bootorabi Fatemeh, Khorramizadeh Mohammad Reza
Department of Aquaculture Science, Babol Branch, Islamic Azad University, Babol, Iran.
Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
J Diabetes Metab Disord. 2022 Jan 27;21(1):823-833. doi: 10.1007/s40200-021-00964-3. eCollection 2022 Jun.
Zebrafish () is an established model for studying various metabolic diseases. The aim of this study was to evaluate the effect of resveratrol as a natural polyphenol on reducing inflammation caused by hyperglycemia (diabetes) and its effect on digestive tissue as well as TNF-α, IFN-γ, and INL1β genes in zebrafish.
Within a 20-day period, the research was performed on 120 adult zebrafish, which were randomly classified into eight groups: two experimental treatments (induced glucose = +G) and (without glucose = -G), where each main group was as follows: CTRL = control and RSV resveratrol with doses 10, 20, and 30 μmol/L. At the end of the period, the blood glucose level was measured using glucose test strip, staining of intestinal tissue was done by hematoxylin and eosin (H&E), and expression of INF-γ, IL1-β, and TNF-α genes extracted from the intestinal was measured via internal method RT-PCR. Data analysis in this study was performed using SPSS software version 21. One-way ANOVA and mean comparison of treatments by Duncan test were used for data analysis. All statistical analyses were performed at a significant level (P < 0.5) where the mean data were presented with standard deviation.
According to the results, the lowest blood sugar level at the end of the experiment belonged to the group (G-RSV20) where no significant difference was observed between treatments (P > 0.05). The highest expression of IL1-β gene belonged to the (G + CTRL) group (P < 0.05), while the (G + RSV20) group showed the lowest expression of the INF-γ gene and had a significant difference with other groups (P < 0.05). In (G + RSV10) treatment, the lowest expression of TNF-α gene was observed and there was no significant difference with other treatments (P > 0.05). Resveratrol would improve the absorption of nutrients in the intestinal tissue by increasing the number of goblet cells as well as the width and height of the villi.
Collectively, this study indicated that treatment with resveratrol could improve metabolic-mediated performances by reducing blood glucose, increasing food absorption in the intestine tissue, and reducing the expression of inflammatory genes in type 2 diabetic zebrafish model.
斑马鱼是研究各种代谢性疾病的成熟模型。本研究旨在评估天然多酚白藜芦醇对减轻高血糖（糖尿病）引起的炎症的作用及其对斑马鱼消化组织以及肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和白细胞介素1β（IL1β）基因的影响。
在20天内，对120条成年斑马鱼进行研究，随机分为八组：两个实验处理组（诱导葡萄糖组=+G）和（无葡萄糖组=-G），每个主要组如下：对照组=CTRL以及剂量为10、20和30 μmol/L的白藜芦醇组（RSV）。在实验期结束时，使用血糖仪测量血糖水平，用苏木精和伊红（H&E）对肠道组织进行染色，并通过内参法逆转录聚合酶链反应（RT-PCR）测量从肠道提取的IFN-γ、IL1-β和TNF-α基因的表达。本研究中的数据分析使用SPSS 21软件进行。采用单因素方差分析和邓肯检验对处理组进行均值比较以进行数据分析。所有统计分析均在显著性水平（P<0.5）下进行，均值数据以标准差表示。
根据结果，实验结束时血糖水平最低的是（G-RSV20）组，各处理组之间未观察到显著差异（P>0.05）。IL1-β基因表达最高的是（G+CTRL）组（P<0.05），而（G+RSV20）组IFN-γ基因表达最低，并与其他组有显著差异（P<0.05）。在（G+RSV10）处理中，观察到TNF-α基因表达最低，与其他处理无显著差异（P>0.05）。白藜芦醇可通过增加杯状细胞数量以及绒毛的宽度和高度来改善肠道组织对营养物质的吸收。
总体而言，本研究表明，在2型糖尿病斑马鱼模型中，白藜芦醇治疗可通过降低血糖、增加肠道组织对食物的吸收以及降低炎症基因的表达来改善代谢介导的性能。
