School of Food and Biological Engineering, Xihua University, Chengdu, P. R. China.
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, P. R. China.
J Food Sci. 2022 Jul;87(7):2999-3012. doi: 10.1111/1750-3841.16207. Epub 2022 Jun 8.
As the main component of truffles, polysaccharides have a variety of biological activities such as anti-oxidation, anti-tumor, and hypoglycemic activity, and these activities are closely related to its structure. In this study, Tuber Aestivum crude polysaccharide (TACP) and Tuber Melanosporum crude polysaccharide (TMCP) were obtained from Tuber Aestivum and Tuber Melanosporum by using microwave-assisted hot water, and then the Sephadex G-200 column was utilized to further separate and purify Tuber Aestivum polysaccharide (TAP) and Tuber Melanosporum polysaccharide (TMP) from TACP and TMCP. The structural characterization results showed that the molecular weight of TAP was 2.18 × 10 kDa, while TMP was 8.79 × 10 kDa. Although the two polysaccharide components were mainly composed of mannose (Man) and glucose (Glc), the molar ratio of Man and Glc in TAP was 14.76: 12.31, with a molar ratio of 5.43:10.94 in TMP. Furthermore, the antioxidant activity of two polysaccharide components was evaluated. TAP and TMP could protect porcine jejunal epithelial (IPEC-J2) cells from oxidative damage by H O , but TAP exhibited stronger antioxidant effects. It was mainly reflected that TAP could increase the secretion level of intracellular antioxidant enzymes (superoxide dismutase and catalase) in IPEC-J2 cells, and had a significant effect on the total antioxidant capacity of cells. The reactive oxygen species and malondialdehyde had better scavenging ability at the concentration of 20 µg/ml. The difference between TAP and TMP may be due to the dissimilar structure. Its structure-activity relationship needs further study. PRACTICAL APPLICATION: The structure of TAP and TMP were different, and TAP had higher molecular weight. Besides, TAP and TMP can protect IPEC-J2 cells from oxidative stress, providing a theoretical basis for developing potential antioxidant drugs of practical significance.
作为块菌的主要成分，多糖具有多种生物活性，如抗氧化、抗肿瘤和降血糖活性，这些活性与其结构密切相关。本研究采用微波辅助热水法从块菌中提取块菌粗多糖（TACP）和块菌粗多糖（TMCP），然后用 Sephadex G-200 柱进一步分离纯化 TACP 和 TMCP 中的块菌多糖（TAP）和块菌多糖（TMP）。结构表征结果表明，TAP 的分子量为 2.18×10 kda，而 TMP 的分子量为 8.79×10 kda。虽然两种多糖成分主要由甘露糖（Man）和葡萄糖（Glc）组成，但 TAP 中 Man 和 Glc 的摩尔比为 14.76:12.31，TMP 中 Man 和 Glc 的摩尔比为 5.43:10.94。此外，还评价了两种多糖成分的抗氧化活性。TAP 和 TMP 可以保护猪空肠上皮（IPEC-J2）细胞免受 H 2 O 2 引起的氧化损伤，但 TAP 表现出更强的抗氧化作用。这主要体现在 TAP 可以增加 IPEC-J2 细胞内抗氧化酶（超氧化物歧化酶和过氧化氢酶）的分泌水平，对细胞的总抗氧化能力有显著影响。在 20 µg/ml 的浓度下，对活性氧和丙二醛有更好的清除能力。TAP 和 TMP 的差异可能是由于结构不同。其构效关系需要进一步研究。实际应用：TAP 和 TMP 的结构不同，TAP 的分子量较高。此外，TAP 和 TMP 可以保护 IPEC-J2 细胞免受氧化应激，为开发具有实际意义的潜在抗氧化药物提供了理论依据。
