Vargas-Bermudez Diana S, Mogollón José Darío, Jaime Jairo
Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Departamento de Salud Animal, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Carrera 30 No. 45-03, Bogotá CP 111321, Colombia.
Pathogens. 2022 May 31;11(6):633. doi: 10.3390/pathogens11060633.
Four genotypes of circovirus have been recognized in swine, with PCV2 and PCV3 being the most associated with clinical manifestations, while PCV4 does not have a defined disease. In addition, PCV2 is associated with different syndromes grouped as diseases associated with porcine circovirus (PCVAD), while PCV3 causes systemic and reproductive diseases. In the present study, we retrospectively detected PCV2, PCV3, and PCV4 in Colombia during two periods: A (2015-2016) and B (2018-2019). During period A, we evaluated stool pools from the 32 Colombian provinces, finding a higher prevalence of PCV3 compared to PCV2 as well as PCV2/PCV3 co-infection. Furthermore, we determined that PCV3 had been circulating since 2015 in Colombia. Regarding period B, we evaluated sera pools and tissues from abortions and stillborn piglets from the five provinces with the highest pig production. The highest prevalence found was for PCV3 in tissues followed by sera pools, while PCV2 was lower and only in sera pools. In addition, PCV2/PCV3 co-infection in sera pools was also found for this period. The complete genome sequences of PCV3 and PCV3-ORF2 placed the Colombian isolates within clade 1 as the majority in the world. For PCV2, the predominant genotype currently in Colombia is PCV2d. Likewise, in some PCV3-ORF2 sequences, a mutation (A24V) was found at the level of the Cap protein, which could be involved in PCV3 immunogenic recognition. Regarding PCV4, retrospective surveillance showed that there is no evidence of the presence of this virus in Colombia.
在猪身上已识别出四种圆环病毒基因型，其中猪圆环病毒2型（PCV2）和猪圆环病毒3型（PCV3）与临床表现关联最为密切，而猪圆环病毒4型（PCV4）尚无明确相关疾病。此外，PCV2与归类为猪圆环病毒相关疾病（PCVAD）的不同综合征有关，而PCV3会引发全身性疾病和繁殖障碍疾病。在本研究中，我们回顾性检测了哥伦比亚在两个时期（A期：2015 - 2016年；B期：2018 - 2019年）的PCV2、PCV3和PCV4。在A期，我们评估了来自哥伦比亚32个省份的粪便样本池，发现PCV3的流行率高于PCV2，以及PCV2/PCV3共同感染情况。此外，我们确定PCV3自2015年起就在哥伦比亚传播。关于B期，我们评估了来自五个生猪产量最高省份的血清样本池以及流产和死产仔猪的组织样本。发现组织中PCV3的流行率最高，其次是血清样本池，而PCV2的流行率较低且仅存在于血清样本池中。此外，该时期血清样本池中也发现了PCV2/PCV3共同感染情况。PCV3和PCV3 - ORF2的全基因组序列表明，哥伦比亚分离株与世界上大多数分离株一样，属于进化枝1。对于PCV2，目前在哥伦比亚占主导地位的基因型是PCV2d。同样，在一些PCV3 - ORF2序列中，发现Cap蛋白水平存在一个突变（A24V），这可能与PCV3的免疫原性识别有关。关于PCV4，回顾性监测表明，在哥伦比亚没有该病毒存在的证据。
