Sharma Ruchira, Pielstick Brittany A, Bell Kimberly A, Nieman Tanner B, Stubbs Olivia A, Yeates Edward L, Baltrus David A, Grose Julianne H
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States.
School of Plant Sciences, The University of Arizona, Tucson, AZ, United States.
Front Microbiol. 2019 Jul 23;10:1533. doi: 10.3389/fmicb.2019.01533. eCollection 2019.
is a plant pathogen from the family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect and rather than bacteria by protein similarity, however, we were only able to detect infection of and the closely related strains of .
是来自该家族的一种植物病原体，也是毁灭性农业病害火疫病的病原体。在这里，我们描述了从美国犹他州瓦萨奇前线分离出的8种的裂解性噬菌体，它们与在加拿大安大略省分离出的vB_EamM_Ea35-70高度相似。这些噬菌体的基因组大小在271至275 kb之间，属于一个新的巨型噬菌体家族。通过基因组和蛋白质组比较、质谱分析、宿主范围和爆发大小对这些巨型噬菌体进行了进一步表征。它们的蛋白质组研究较少，有超过200种推定蛋白质没有已知的同源物。通过质谱分析证实了其中27种推定蛋白质的产生。从蛋白质相似性来看，这些噬菌体似乎与感染和的噬菌体最为相似，而不是感染细菌的噬菌体，然而，我们只能检测到对和的密切相关菌株的感染。
