Animal Science and Technology College, Beijing University of Agriculture, Beijing, China.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Biol Reprod. 2022 Jul 25;107(1):226-236. doi: 10.1093/biolre/ioac119.
Pluripotent stem cells (PSCs) harbor the capacity of unlimited self-renewal and multilineage differentiation potential, which are crucial for basic research and biomedical science. Establishment of PSCs with defined features was previously reported from mice and humans, while generation of stable large animal PSCs has experienced a relatively long trial stage and only recently has made breakthroughs. Pigs are regarded as ideal animal models for their similarities in physiology and anatomy to humans. Generation of porcine PSCs would provide cell resources for basic research, genetic engineering, animal breeding, and cultured meat. In this review, we summarize the progress on the derivation of porcine PSCs and reprogramed cells and elucidate the mechanisms of pluripotency changes during pig embryo development. This will be beneficial for understanding the divergence and conservation between different species involved in embryo development and the pluripotent-regulated signaling pathways. Finally, we also discuss the promising future applications of stable porcine PSCs. Even though challenges remain in the field of porcine stem cells, these progress and viewpoints would provide guidance in future research direction.
多能干细胞（PSCs）具有无限自我更新和多能分化潜能，这对于基础研究和生物医学科学至关重要。此前已经有报道称，从老鼠和人类中分离出了具有特定特征的 PSCs，而稳定的大型动物 PSCs 的产生经历了一个相对较长的试验阶段，直到最近才取得突破。猪因其在生理学和解剖学上与人类的相似性而被认为是理想的动物模型。猪 PSCs 的产生将为基础研究、基因工程、动物繁殖和培养肉提供细胞资源。在这篇综述中，我们总结了猪 PSCs 和重编程细胞的衍生进展，并阐明了猪胚胎发育过程中多能性变化的机制。这将有助于理解不同物种在胚胎发育过程中的差异和保守性，以及多能调控信号通路。最后，我们还讨论了稳定的猪 PSCs 的有前途的未来应用。尽管猪干细胞领域仍然存在挑战，但这些进展和观点将为未来的研究方向提供指导。
