Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Campus Eure et Loir, Orleans University, 28000 Chartres, France.
Molecules. 2022 Jun 2;27(11):3590. doi: 10.3390/molecules27113590.
L. is a potential plant in the Nymphaeaceae family that is well-recognized as an economic and traditional medicinal plant in Thailand and other countries. Its pharmacological and medicinal effects have been confirmed. However, there is no study going deeper into the population level to examine the phytochemical variation and biological activity of each population that benefits phytopharmaceutical and medical applications using this plant as raw material. This study was intensely conducted to complete this important research gap to investigate the flavonoid profiles from its floral parts, the stamen and perianth, as well as the antioxidant potential of the 13 populations collected from every floristic region by (1) analyzing their flavonoid profiles, including the HPLC analysis, and (2) investigating the antioxidant capacity of these populations using three assays to observe different antioxidant mechanisms. The results indicated that the northeastern and northern regions are the most abundant floristic regions, and flavonoids are the main phytochemical class of both stamen and perianth extracts from The stamen offers higher flavonoids and richer antioxidant potential compared with the perianth. This finding is also the first completed report at the population level to describe the significant correlation between the phytochemical profiles in floral parts extracts and the main antioxidant activity, which is mediated by the electron transfer mechanism. The results from the Pearson correlation coefficients between several phytochemicals and different antioxidant assessments highlighted that the antioxidant capability of these extracts is the result of complex phytochemical combinations. The frontier knowledge from these current findings helps to open up doors for phytopharmaceutical industries to discover their preferred populations and floral parts that fit with their targeted products.
L. 是睡莲科的一种有潜力的植物，在泰国和其他国家被广泛认为是一种经济和传统的药用植物。其药理和药用功效已得到证实。然而，目前还没有深入到种群水平的研究来检查每个种群的植物化学变异和生物活性，而这些种群的植物化学变异和生物活性可以利用这种植物作为原料来促进植物药物和医学应用。本研究旨在深入研究这一重要的研究空白，以调查其花部（雄蕊和花瓣）的类黄酮谱，以及从每个植物区系收集的 13 个种群的抗氧化潜力，方法是（1）分析它们的类黄酮谱，包括 HPLC 分析，以及（2）使用三种测定方法来研究这些种群的抗氧化能力，以观察不同的抗氧化机制。结果表明，东北地区和北部地区是最丰富的植物区系，类黄酮是雄蕊和花瓣提取物的主要植物化学类，与花瓣相比，雄蕊提供了更高的类黄酮和更丰富的抗氧化潜力。这一发现也是首次在种群水平上完成的报告，描述了花部提取物的植物化学特征与主要抗氧化活性之间的显著相关性，这种相关性是由电子转移机制介导的。几种植物化学物质与不同抗氧化评估之间的 Pearson 相关系数的结果强调了这些提取物的抗氧化能力是复杂的植物化学组合的结果。这些当前发现的前沿知识有助于为植物药物行业开辟道路，以发现适合其目标产品的首选种群和花部。
