（Wall. ex Benth.）Codd 的离体培养物：建立、植物化学特征以及体外抗氧化和抗衰老活性。

(Wall. ex Benth.) Codd In Vitro Cultures: Establishment, Phytochemical Characterization and In Vitro Antioxidant and Anti-Aging Activities.

机构信息

Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.

Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC EA1207), INRA USC1328, Plant Lignans Team, Université d'Orléans, 45067 Orléans CÉDEX 2, France.

出版信息

Int J Mol Sci. 2019 Jan 21;20(2):452. doi: 10.3390/ijms20020452.

(Wall. ex Benth.) Codd accumulates large amounts of phenolics and pentacyclic triterpenes. The present study deals with the in vitro callus induction from stem and leaf explants of under various plant growth regulators (PGRs) for the production of antioxidant and anti-ageing compounds. Among all the tested PGRs, thidiazuron (TDZ) used alone or in conjunction with α-napthalene acetic acid (NAA) induced highest callogenesis in stem-derived explants, as compared to leaf-derived explants. Stem-derived callus culture displayed maximum total phenolic content and antioxidant activity under optimum hormonal combination (3.0 mg/L TDZ + 1.0 mg/L NAA). HPLC analysis revealed the presence of plectranthoic acid (373.92 µg/g DW), oleanolic acid (287.58 µg/g DW), betulinic acid (90.51 µg/g DW), caffeic acid (91.71 µg/g DW), and rosmarinic acid (1732.61 µg/g DW). Complete antioxidant and anti-aging potential of extracts with very contrasting phytochemical profiles were investigated. Correlation analyses revealed rosmarinic acid as the main contributor for antioxidant activity and anti-aging hyaluronidase, advance glycation end-products inhibitions and SIRT1 activation, whereas, pentacyclic triterpenoids were correlated with elastase, collagenase, and tyrosinase inhibitions. Altogether, these results clearly evidenced the great valorization potential of calli for the production of antioxidant and anti-aging bioactive extracts for cosmetic applications.

（Wall. ex Benth.）Codd 积累了大量的酚类和五环三萜。本研究涉及在不同植物生长调节剂（PGRs）下从茎和叶外植体诱导的愈伤组织，以生产抗氧化和抗衰老化合物。在所有测试的 PGRs 中，噻二唑脲（TDZ）单独使用或与 α-萘乙酸（NAA）联合使用，与叶外植体相比，诱导茎外植体产生最高的愈伤组织。在最佳激素组合（3.0 mg/L TDZ + 1.0 mg/L NAA）下，茎衍生的愈伤组织培养显示出最大的总酚含量和抗氧化活性。HPLC 分析显示存在刺桐酸（373.92 µg/g DW）、齐墩果酸（287.58 µg/g DW）、桦木酸（90.51 µg/g DW）、咖啡酸（91.71 µg/g DW）和迷迭香酸（1732.61 µg/g DW）。研究了具有非常不同植物化学特征的提取物的完整抗氧化和抗衰老潜力。相关性分析表明，迷迭香酸是抗氧化活性和抗衰老透明质酸酶、糖基化终产物抑制和 SIRT1 激活的主要贡献者，而五环三萜类化合物与弹性蛋白酶、胶原酶和酪氨酸酶抑制有关。总之，这些结果清楚地证明了愈伤组织在生产抗氧化和抗衰老生物活性提取物方面具有巨大的增值潜力，可用于化妆品应用。