云芝酸，从云芝中提取的三萜类化合物，抑制胃癌细胞的侵袭和转移。
Poria Acid, Triterpenoids Extracted from , Inhibits the Invasion and Metastasis of Gastric Cancer Cells.
机构信息
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China.
The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
出版信息
Molecules. 2022 Jun 6;27(11):3629. doi: 10.3390/molecules27113629.
BACKGROUND
is an important medicinal fungus in traditional Chinese medicine. Poria acid (PA), a triterpenoid compound, is an effective component of traditional Chinese medicine . This experiment investigated the anti-gastric cancer biological activity of PA in vitro.
METHODS
The effect of PA on the viability of gastric cancer cells was detected by the thiazolyl blue (MTT) assay. Cell adhesion assays were used to detect changes in the adhesion of cells treated after PA (0, 20, 40, and 80 µmol/L). The ability of cell invasion and migration were detected by Transwell assays and wound healing assays. A high-content imaging system was used to dynamically record the motility of the gastric cancer cells after PA (0, 20, 40, and 80 µmol/L) treatment. Western blotting was used to detect the expression of epithelial-mesenchymal transformation (EMT), invasion and migration related proteins.
RESULTS
The MTT assay showed that the proliferation of gastric cancer cells was significantly inhibited after PA treatment. Cell adhesion experiments showed that the adhesion of gastric cancer cells was significantly decreased after PA treatment. Compared with the control group, the wound healing area of the gastric cancer cells treated with different concentrations of PA decreased. The Transwell assay showed that the number of gastric cancer cells passing through the cell membrane were significantly reduced after PA treatment. In addition, after PA treatment, the cells' movement distance and average movement speed were significantly lower than those of the control group. Finally, PA can significantly alter the expression of EMT-related proteins E-cadherin, N-cadherin, and Vimentin and decreased the expressions of metastasis-related proteins matrix metalloproteinase (MMP) 2, MMP-9 and tissue inhibition of matrix metalloproteinase (TIMP)1 in the gastric cancer cells.
CONCLUSIONS
Triterpenoids from have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.
背景
是传统中药中的一种重要药用真菌。 ，一种三萜类化合物，是传统中药的有效成分。本实验研究了 PA 在体外的抗胃癌生物活性。
方法
噻唑蓝（MTT）法检测 PA 对胃癌细胞活力的影响。细胞黏附实验检测 PA（0、20、40 和 80μmol/L）处理后细胞黏附的变化。Transwell 实验和划痕愈合实验检测细胞侵袭和迁移能力。高内涵成像系统动态记录 PA（0、20、40 和 80μmol/L）处理后胃癌细胞的运动。Western blot 检测上皮-间质转化（EMT）、侵袭和迁移相关蛋白的表达。
结果
MTT 实验表明，PA 处理后胃癌细胞的增殖明显受到抑制。细胞黏附实验表明，PA 处理后胃癌细胞的黏附明显减少。与对照组相比，不同浓度 PA 处理的胃癌细胞的愈合面积减小。Transwell 实验表明，PA 处理后穿过细胞膜的胃癌细胞数量明显减少。此外，PA 处理后，细胞的运动距离和平均运动速度明显低于对照组。最后，PA 可明显改变 EMT 相关蛋白 E-钙黏蛋白、N-钙黏蛋白和波形蛋白的表达，并降低胃癌细胞中转移相关蛋白基质金属蛋白酶（MMP）2、MMP-9 和组织抑制金属蛋白酶（TIMP）1 的表达。
结论
来源于 的三萜类化合物对胃癌具有显著的生物学活性，其机制可能涉及上皮-间质转化过程。
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