College of Science, Tibet University, Lhasa 850001, China.
School of Life Sciences, Yunnan University, Kunming 650106, China.
Molecules. 2022 Jun 6;27(11):3645. doi: 10.3390/molecules27113645.
The authors of this paper conducted a comparative metabolomic analysis of (OS), providing the metabolic profiles of the stroma (OSBSz) and sclerotia (OSBSh) of OS by widely targeted metabolomics and untargeted metabolomics. The results showed that 778 and 1449 metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. The metabolites in OSBSz and OSBSh are significantly differentiated; 71 and 96 differentially expressed metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. This suggests that these 71 metabolites (riboflavine, tripdiolide, bromocriptine, lumichrome, tetrahymanol, citrostadienol, etc.) and 96 metabolites (sancycline, vignatic acid B, pirbuterol, rubrophen, epalrestat, etc.) are potential biomarkers. 4-Hydroxybenzaldehyde, arginine, and lumichrome were common differentially expressed metabolites. Using the widely targeted metabolomics approach, the key pathways identified that are involved in creating the differentiation between OSBSz and OSBSh may be nicotinate and nicotinamide metabolism, thiamine metabolism, riboflavin metabolism, glycine, serine, and threonine metabolism, and arginine biosynthesis. The differentially expressed metabolites identified using the untargeted metabolomics approach were mainly involved in arginine biosynthesis, terpenoid backbone biosynthesis, porphyrin and chlorophyll metabolism, and cysteine and methionine metabolism. The purpose of this research was to provide support for the assessment of the differences between the stroma and sclerotia, to furnish a material basis for the evaluation of the physical effects of OS, and to provide a reference for the selection of detection methods for the metabolomics of OS.
本文作者对 （OS）进行了比较代谢组学分析，通过广泛靶向代谢组学和非靶向代谢组学为 OS 的基质（OSBSz）和菌核（OSBSh）提供了代谢图谱。结果表明，广泛靶向代谢组学和非靶向代谢组学方法分别鉴定出 778 种和 1449 种代谢物。OSBSz 和 OSBSh 中的代谢物存在显著差异；通过广泛靶向代谢组学和非靶向代谢组学方法分别鉴定出 71 种和 96 种差异表达代谢物。这表明这些 71 种代谢物（核黄素、三萜二内酯、溴隐亭、光黄素、四氢大麻酚、茄啶烯醇等）和 96 种代谢物（桑辛碱、 vignatic 酸 B、吡布特罗、rubrophen、依帕司他等）可能是潜在的生物标志物。4-羟基苯甲醛、精氨酸和光黄素是常见的差异表达代谢物。使用广泛靶向代谢组学方法，确定的关键途径可能涉及烟酰胺和烟酸代谢、硫胺素代谢、核黄素代谢、甘氨酸、丝氨酸和苏氨酸代谢以及精氨酸生物合成，这些途径涉及 OSBSz 和 OSBSh 之间的分化。使用非靶向代谢组学方法鉴定的差异表达代谢物主要涉及精氨酸生物合成、萜类骨架生物合成、卟啉和叶绿素代谢以及半胱氨酸和蛋氨酸代谢。本研究旨在为评估基质和菌核之间的差异提供支持，为评价 OS 的物理效应提供物质基础，并为 OS 代谢组学检测方法的选择提供参考。
