Institute for Nanostructures, Nanomodelling and Nanofabrication (i3N), Physics Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Radiation Imaging Technologies Lda. (RI-TE), University of Aveiro Incubator, PCI-Creative Science Park, 3830-352 Ílhavo, Portugal.
Sensors (Basel). 2022 Jun 3;22(11):4264. doi: 10.3390/s22114264.
Monitoring the vital signs of mice is an essential practice during imaging procedures to avoid populational losses and improve image quality. For this purpose, a system based on a set of devices (piezoelectric sensor, optical module and thermistor) able to detect the heart rate, respiratory rate, body temperature and arterial blood oxygen saturation (SpO2) in mice anesthetized with sevoflurane was implemented. Results were validated by comparison with the reported literature on similar anesthetics. A new non-invasive electrocardiogram (ECG) module was developed, and its first results reflect the viability of its integration in the system. The sensors were strategically positioned on mice, and the signals were acquired through a custom-made printed circuit board during imaging procedures with a micro-PET (Positron Emission Tomography). For sevoflurane concentration of 1.5%, the average values obtained were: 388 bpm (beats/minute), 124 rpm (respirations/minute) and 88.9% for the heart rate, respiratory rate and SpO2, respectively. From the ECG information, the value obtained for the heart rate was around 352 bpm for injectable anesthesia. The results compare favorably to the ones established in the literature, proving the reliability of the proposed system. The ECG measurements show its potential for mice heart monitoring during imaging acquisitions and thus for integration into the developed system.
监测小鼠的生命体征是在成像过程中避免种群损失和提高图像质量的必要实践。为此，我们开发了一种基于一组设备（压电传感器、光学模块和热敏电阻）的系统，该系统能够检测麻醉后使用七氟醚的小鼠的心率、呼吸频率、体温和动脉血氧饱和度（SpO2）。结果通过与类似麻醉剂的文献报道进行比较得到验证。我们还开发了一种新的非侵入式心电图（ECG）模块，并通过在 micro-PET（正电子发射断层扫描）中进行成像过程，首次获得了其集成到系统中的可行性结果。传感器被战略性地放置在小鼠身上，并通过定制的印刷电路板在成像过程中获取信号。对于七氟醚浓度为 1.5%，我们获得的平均数值分别为：388 bpm（每分钟心跳）、124 rpm（每分钟呼吸）和 88.9%的心率、呼吸率和 SpO2。从 ECG 信息中，我们得到的注射麻醉后的心率约为 352 bpm。结果与文献中的结果相当，证明了所提出系统的可靠性。ECG 测量显示了其在成像采集期间监测小鼠心脏的潜力，因此可集成到开发的系统中。
