Huo Rui, Zhang Meili, Zhang Yakun, Bai Xue, Zhang Yuanyuan, Guo Xinyue
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.
Front Nutr. 2022 May 24;9:871556. doi: 10.3389/fnut.2022.871556. eCollection 2022.
Using oat-corn-konjac extruded mixed powder, oat bran micro powder, skim milk powder, Pueraria whole powder, and pumpkin powder as raw materials, a formula powder with high dietary fiber was prepared, and its effect on obesity in mice with a high-fat diet was investigated. After 7 days of adaptive feeding, the mice were divided into blank group, high-fat diet group, formula powder + high-fat diet group, and weight-loss drug + high-fat diet group. After 8 weeks of treatment, the body weight of mice were observed and measured to determine the composition of tract flora, liver leptin content, insulin content, and activities of AMP-activated protein kinase (AMPK), lipoprotein lipase (LPL), fatty acid synthetase (FAS), sterol-regulatory element-binding proteins (SREBPs), and acetyl CoA carboxylase 1 (ACC1). The results indicated that treatment with the formula powder could reduce the body weight of mice and increase the abundance of , and compared to the group given a high-fat diet. Moreover, the leptin and insulin contents of the experimental group decreased from 5.67 μg/L to 0.12 μg/L and from 12.71 μg/L to 7.13 μg/L, respectively, compared to the control group, which was not significantly different from the blank group (P > 0.05). Also, the activities of AMPK and LPL increased, and the activities of FAS, SREBPs, and ACC1 were significantly decreased (P < 0.05). Some pathogenic bacteria were significantly positively correlated with leptin and FAS and significantly negatively correlated with LPL. Some beneficial bacteria were positively correlated with LPL. Therefore, the formula powder used in this study could reduce the body weight of mice, increase the abundance of some beneficial bacteria in the colonic intestinal microbiota, and improve the activities of enzymes related to lipid metabolism in the liver. This study provides a theoretical reference for the pathway by which high-fiber diet improves liver and intestinal metabolic abnormalities.
以燕麦 - 玉米 - 魔芋挤压混合粉、燕麦麸微粉、脱脂奶粉、葛根全粉和南瓜粉为原料，制备一种高膳食纤维配方粉，并研究其对高脂饮食小鼠肥胖的影响。适应性喂养7天后，将小鼠分为空白组、高脂饮食组、配方粉 + 高脂饮食组和减肥药 + 高脂饮食组。治疗8周后，观察并测量小鼠体重，测定肠道菌群组成、肝脏瘦素含量、胰岛素含量以及AMP激活蛋白激酶（AMPK）、脂蛋白脂肪酶（LPL）、脂肪酸合成酶（FAS）、固醇调节元件结合蛋白（SREBPs）和乙酰辅酶A羧化酶1（ACC1）的活性。结果表明，与高脂饮食组相比，配方粉处理可降低小鼠体重，并增加[此处原文缺失部分菌群名称]的丰度。此外，与对照组相比，实验组的瘦素和胰岛素含量分别从5.67μg/L降至0.12μg/L和从12.71μg/L降至7.13μg/L，与空白组无显著差异（P>0.05）。同时，AMPK和LPL的活性增加，FAS、SREBPs和ACC1的活性显著降低（P<0.05）。一些病原菌与瘦素和FAS呈显著正相关，与LPL呈显著负相关。一些有益菌与LPL呈正相关。因此，本研究中使用的配方粉可降低小鼠体重，增加结肠肠道微生物群中一些有益菌的丰度，并改善肝脏中与脂质代谢相关酶的活性。本研究为高纤维饮食改善肝脏和肠道代谢异常的途径提供了理论参考。
