绿茶提取物制备的氧化锰纳米颗粒的生物合成及其抗菌活性

Biosynthesis and antibacterial activity of manganese oxide nanoparticles prepared by green tea extract.

作者信息

Saod Wahran M, Hamid Layth L, Alaallah Nisreen Jassam, Ramizy Asmiet

机构信息

Chemistry department, College of science, University Of Anbar, Ramadi, Iraq.

Biology department, College of science, University Of Anbar, Ramadi, Iraq.

出版信息

Biotechnol Rep (Amst). 2022 Apr 11;34:e00729. doi: 10.1016/j.btre.2022.e00729. eCollection 2022 Jun.

Amongst chemical and physical techniques, the biosynthesis method of metal nanoparticles has received the interest of many researchers owing to its environmental safety, simplicity and inexpensiveness. Manganese oxide nanoparticles (MnO NPs) were successfully synthesised using green tea extract as the reducing agent and characterised by UV-Vis spectroscopy, X-ray diffractometry and Fourier transform infrared spectroscopy. The shape and size of the MnO NPs were obtained by scanning electron microscopy. The size of the MnO NPs was 20-30 nm. The MnO NPs exhibited strong antibacterial activity against pathogenic bacteria, namely, and , with inhibition zones of 12, 14 and 18 mm, respectively. Moreover, the minimum inhibitory concentration (MIC) of the MnO NPs was 12.5 U/mL as determined by resazurin microtitre assay. The activities of some antibiotics remarkably increased when combined with MnO NPs (at MIC).

在化学和物理技术中，金属纳米颗粒的生物合成方法因其环境安全性、简便性和低成本而受到众多研究人员的关注。使用绿茶提取物作为还原剂成功合成了氧化锰纳米颗粒（MnO NPs），并通过紫外可见光谱、X射线衍射和傅里叶变换红外光谱对其进行了表征。通过扫描电子显微镜获得了MnO NPs的形状和尺寸。MnO NPs的尺寸为20 - 30纳米。MnO NPs对病原菌即[此处原文缺失病原菌名称]表现出很强的抗菌活性，抑菌圈分别为12、14和18毫米。此外，通过刃天青微量滴定法测定，MnO NPs的最低抑菌浓度（MIC）为12.5 U/mL。当与MnO NPs（在MIC浓度下）联合使用时，一些抗生素的活性显著增强。