Rahimi Mahsa, Khanahmad Hossein, Gharipour Mojgan, Roohafza Hamidreza, Dianatkhah Minoo, Khosravi Elham, Sadeghian Ladan, Sadeghi Masoumeh
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics and Epigenetics, Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
ARYA Atheroscler. 2021 Sep;17(5):1-8. doi: 10.22122/arya.v17i0.2369.
Myocardial infarction (MI) is one of the leading causes of mortality globally. Although it is most prevalent in the elderly, it may occur in young adults (men ≤ 55 years or women ≤ 65 years) as premature MI (PMI). As awareness of genetic risks may lead to effective prevention of PMI, we aim to investigate the association of two susceptible single nucleotide polymorphisms (SNPs) in the LPA gene with PMI in the Iranian population, rs1801693 and rs7765781, identified in previous genome-wide association studies (GWAS).
A total number of 85 patients with PMI and 85 healthy controls were recruited from December 2015 to March 2016 from Isfahan, Iran. Peripheral blood samples were collected from all individuals. Deoxyribonucleic acid (DNA) was extracted and genotyped at rs1181693 and rs7765781 polymorphisms, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results were statistically analyzed to find any possible association of the two polymorphisms with PMI by SPSS software and P-values less than 0.05 were considered to be statistically significant.
Statistical analysis displayed no significant difference between rs1801693 (P = 0.815)/rs7765781 (P = 0.746) alleles in patients with PMI and healthy control subjects.
There is no meaningful association between rs1801693/rs7765781 and PMI incidence in the Iranian population.
心肌梗死（MI）是全球主要的死亡原因之一。尽管它在老年人中最为常见，但也可能发生在年轻人（男性≤55岁或女性≤65岁）中，即早发性心肌梗死（PMI）。由于对遗传风险的认识可能有助于有效预防PMI，我们旨在研究伊朗人群中LPA基因的两个易感单核苷酸多态性（SNP），即rs1801693和rs7765781（在先前的全基因组关联研究（GWAS）中确定）与PMI之间的关联。
2015年12月至2016年3月，从伊朗伊斯法罕招募了85例PMI患者和85名健康对照。从所有个体采集外周血样本。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法提取脱氧核糖核酸（DNA），并对rs1181693和rs7765781多态性进行基因分型。通过SPSS软件对结果进行统计分析，以发现这两个多态性与PMI之间的任何可能关联，P值小于0.05被认为具有统计学意义。
统计分析显示，PMI患者和健康对照受试者的rs1801693（P = 0.815）/rs7765781（P = 0.746）等位基因之间无显著差异。
在伊朗人群中，rs1801693/rs7765781与PMI发病率之间没有有意义的关联。
