Division of Periodontology, Department of Developmental and Surgical Sciences, University of Minnesota, Minneapolis, MN, USA -
Department of Preventive Dentistry, Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece -
Minerva Dent Oral Sci. 2022 Dec;71(6):329-338. doi: 10.23736/S2724-6329.22.04709-X. Epub 2022 Jun 10.
Although non-surgical periodontal treatment is considered the gold standard, a subgroup of patients displays recurrence/progression of periodontitis after treatment. The aim of the present prospective study was to assess the effect of IL-6 -572 G/C and IL-10 -592 C/A gene polymorphisms on the risk of disease recurrence/progression at 3 years following non-surgical periodontal treatment.
Thirty-seven patients diagnosed with chronic periodontitis received oral hygiene instructions and non-surgical periodontal treatment and were monitored for 3 years. All individuals were clinically evaluated for PPD, CAL and BOP at baseline and 3 years. Based on the clinical findings at 3 years, all subjects were considered either "at risk" or "not at risk" of periodontal disease progression based on specific criteria. Blood samples were collected at baseline and genotyping of the polymorphisms in IL-6 (rs1800796) and IL-10 (rs1800872) genes were performed by PCR.
Following DNA separation and genotyping, 70.3% of the patients were homozygous carriers of the IL-6 -572G and 45.9% were carriers of the IL-10 -592A allele. Individuals at risk of disease progression ranged from 16.2% to 56.8% based on the criteria used. IL-6 -572 G/C and IL-10 -592 C/A polymorphisms were not associated with an increased risk of further disease progression (P>0.05) when the three criteria were examined. All examined periodontal clinical measures were significantly improved (P<0.05) after treatment. Males showed a significantly higher risk of disease progression than females when full-mouth BOP ≥30% was considered (P=0.008).
Within the limitations of this 3-year prospective study, individuals susceptible to periodontal disease as determined by the presence of the IL-6 -572GG genotype or the IL-10 -592A allele were not associated with an increased risk of further disease progression and the potential need for further treatment following non-surgical periodontal treatment. Males were more prone to be at risk of disease progression than females.
虽然非手术牙周治疗被认为是金标准，但一小部分患者在治疗后会出现牙周炎的复发/进展。本前瞻性研究的目的是评估 IL-6-572G/C 和 IL-10-592C/A 基因多态性对非手术牙周治疗后 3 年内疾病复发/进展风险的影响。
37 名被诊断为慢性牙周炎的患者接受了口腔卫生指导和非手术牙周治疗，并进行了 3 年的监测。所有患者在基线和 3 年均进行了 PPD、CAL 和 BOP 的临床评估。根据 3 年时的临床发现，根据特定标准，所有患者均被认为存在或不存在牙周病进展的风险。在基线时采集血样，并通过 PCR 对 IL-6(rs1800796)和 IL-10(rs1800872)基因的多态性进行基因分型。
在 DNA 分离和基因分型后，70.3%的患者是 IL-6-572G 的纯合子携带者，45.9%的患者是 IL-10-592A 等位基因的携带者。根据使用的标准，疾病进展风险患者的范围为 16.2%至 56.8%。当检查三个标准时，IL-6-572G/C 和 IL-10-592C/A 多态性与进一步疾病进展的风险增加无关(P>0.05)。所有检查的牙周临床指标在治疗后均显著改善(P<0.05)。当全口 BOP≥30%时，男性比女性有更高的疾病进展风险(P=0.008)。
在这项为期 3 年的前瞻性研究的限制内，被确定为 IL-6-572GG 基因型或 IL-10-592A 等位基因的个体易患牙周病，但与进一步疾病进展的风险增加无关，也不需要在非手术牙周治疗后进行进一步治疗。男性比女性更容易发生疾病进展。
