Chatzopoulos G-S, Doufexi A-E, Kouvatsi A
Advanced Education Program in Periodontology, University of Minnesota, 515 Delaware Street SE, Minneapolis, MN 55455, USA,
Med Oral Patol Oral Cir Bucal. 2017 Jul 1;22(4):e446-e457. doi: 10.4317/medoral.21795.
Genetic polymorphisms are commonly associated with altered transcriptional activity and possibly make individuals more susceptible to periodontal disease development, increased disease severity and poor treatment outcome. The study aimed to determine the effect of Interleukin-6 (IL-6) -572 G/C (rs1800796) and IL-10 -592 C/A (rs1800872) polymorphisms on the outcomes of non-surgical periodontal therapy in a Caucasian population.
Sixty-eight patients with chronic periodontal disease were grouped according to their genotype: IL-6, IL-10, IL-6 and IL-10 susceptible (SCP) and non-susceptible (NSCP). All individuals were clinically evaluated at the first visit, and blood sample were collected from patients after checking the inclusion and exclusion criteria of the study. All patients received non-surgical periodontal therapy from a single-blinded periodontist. Clinical periodontal measurements were repeated 45 days after therapy.
This population mean aged 47.63 years included 52.2% females and 58.2% non-smokers. Following DNA separation and genotyping, 65.7% of patients were homozygous carriers of the IL-6 - 572G; 49.3% were carriers of the IL-10 -592A- allele (AA and CA genotypes); and 35.8% carried SCP genotypes for both polymorphisms. The clinical parameters after therapy were not associated with the genotype status. The multiple logistic regression analysis did not show any statistically significant association between the genotypes and the variables tested.
Within the limitations of this longitudinal study, it can be suggested that IL-6 -572 G/C and IL-10 -592 C/A polymorphisms as well as their combination do not influence the outcome of nonsurgical periodontal therapy in Caucasian patients diagnosed with chronic periodontal disease.
基因多态性通常与转录活性改变相关，可能使个体更易患牙周病、疾病严重程度增加及治疗效果不佳。本研究旨在确定白细胞介素-6（IL-6）-572 G/C（rs1800796）和白细胞介素-10 -592 C/A（rs1800872）多态性对高加索人群非手术牙周治疗结果的影响。
68例慢性牙周病患者根据其基因型分组：IL-6、IL-10、IL-6和IL-10易感（SCP）组和非易感（NSCP）组。所有个体在首次就诊时进行临床评估，在检查研究的纳入和排除标准后从患者采集血样。所有患者均由单盲牙周病医生进行非手术牙周治疗。治疗45天后重复进行临床牙周测量。
该人群平均年龄47.63岁，女性占52.2%，非吸烟者占58.2%。经过DNA分离和基因分型后，65.7%的患者是IL-6 - 572G的纯合携带者；49.3%是IL-10 -592A等位基因（AA和CA基因型）的携带者；35.8%的患者两种多态性均携带SCP基因型。治疗后的临床参数与基因型状态无关。多元逻辑回归分析未显示基因型与所测试变量之间有任何统计学上的显著关联。
在本纵向研究的局限性范围内，可以认为IL-6 -572 G/C和IL-10 -592 C/A多态性及其组合不会影响诊断为慢性牙周病的高加索患者非手术牙周治疗的结果。
