Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China.
Talanta. 2022 Nov 1;249:123585. doi: 10.1016/j.talanta.2022.123585. Epub 2022 May 26.
Herein, we described an integrated microfluidic system for multi-target biochemical analysis of micro-volumes of blood samples. This system mainly consists of a microfluid handling module, an integrated microwell array chip, a temperature control module and a spectral detection module. A novel microfluidic plasma extraction approach was developed by coupling the filter membrane-based plasma separation technique with the microfluidic liquid-handling technique. With this approach, quantitative extraction of trace blood with volumes as low as a drop (30 μL) can be automatically realized. Multiple operations in multi-target biochemical analysis are achieved including micro-volume blood collecting, quantitative plasma extraction, plasma dilution, plasma distribution, transferring, biochemical reaction and absorption spectroscopy detection. This system was applied to the multi-target biochemical analysis of glucose, cholesterol and total protein in blood samples and could achieve the determination of the target analytes of a 30-μL blood sample within 11 min. The experimental results are consistent with those obtained by a commercial biochemical analyzer.
在这里，我们描述了一种用于微量血样多靶标生化分析的集成微流控系统。该系统主要由微流处理模块、集成微井阵列芯片、温度控制模块和光谱检测模块组成。通过将基于滤膜的等离子体分离技术与微流控液体处理技术相结合，开发了一种新颖的微流体等离子体提取方法。通过这种方法，可以自动实现体积低至一滴（30 μL）的微量血的定量提取。在多靶标生化分析中实现了多种操作，包括微体积血液采集、定量血浆提取、血浆稀释、血浆分配、转移、生化反应和吸收光谱检测。该系统应用于血液样品中葡萄糖、胆固醇和总蛋白的多靶标生化分析，可在 11 分钟内完成 30 μL 血样中目标分析物的测定。实验结果与商业生化分析仪的结果一致。
