Univ. Bordeaux, EPOC, UMR 5805, F-33600, Pessac, France.
Univ. Bordeaux, EPOC, UMR 5805, F-33600, Pessac, France.
Phytochemistry. 2022 Sep;201:113256. doi: 10.1016/j.phytochem.2022.113256. Epub 2022 Jun 9.
Four undescribed flavonoid sulfates were isolated from Phyllospadix torreyi S. Watson foliar tissue. In addition, nine known flavonoid sulfates and three phenolic acids were isolated from the same extract, of which seven had never been reported for the genus Phyllospadix. Structural elucidation of individual phenolics was assigned using complementary informations from their spectral evidence (HPLC-DAD, LC-MS, NMR, and UV) and chemical behavior. The inter-annual variation in phenolic concentrations was determined by quantitative HPLC-DAD over a three-year period. The results showed a relative constancy of phenolic content over time and the high prevalence of flavonoid disulfates (70-90% of the total flavonoids detected). All samples were found dominated by the unreported nepetin 7, 3'-disulfate and 5-methoxyluteolin 7, 3'-disulfate, followed by luteolin 7, 3'-disulfate. Considering the economic potential of flavonoid sulfates in the pharmaceutical and nutraceutical segments, a sample of detrital leaves was also analyzed. The same phenolic pattern was found and the concentration of the individuals, although lower than in fresh material, makes this abundant biomass of interest for dietary and pharmaceutical applications.
从 Phyllospadix torreyi S. Watson 的叶组织中分离得到了四种未描述的类黄酮硫酸盐。此外，从同一提取物中分离得到了九种已知的类黄酮硫酸盐和三种酚酸，其中七种从未在 Phyllospadix 属中报道过。通过光谱证据（HPLC-DAD、LC-MS、NMR 和 UV）和化学行为的互补信息，确定了各个酚类化合物的结构阐明。通过三年内的定量 HPLC-DAD 测定了酚类浓度的年际变化。结果表明，酚类含量随时间相对稳定，并且二硫酸盐类黄酮的出现频率很高（占检测到的总类黄酮的 70-90%）。所有样品均以未报道的新橙皮苷 7,3'-二硫酸盐和 5-甲氧基木犀草素 7,3'-二硫酸盐为主，其次是木犀草素 7,3'-二硫酸盐。考虑到类黄酮硫酸盐在制药和营养保健品领域的经济潜力，还对碎屑叶片样品进行了分析。发现了相同的酚类模式，尽管个体浓度低于新鲜材料，但这种丰富的生物量对于饮食和药物应用具有重要意义。
