College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu 525-8577, Shiga, Japan.
Research Organization of Science and Technology, Ritsumeikan University, Kusatsu 525-8577, Shiga, Japan.
Molecules. 2024 Oct 15;29(20):4888. doi: 10.3390/molecules29204888.
(Asteraceae) is employed as a hemostatic agent in many traditional medicines, owing to its sulfated flavonoid content. In this study, we obtained crude drug samples from three provinces collected in different years and analyzed their sulfated flavonoid contents using liquid chromatography-mass spectrometry (LC-MS) for quality evaluation. Because sulfated flavonoids are unstable and difficult to isolate from extracts, this study first synthesized a variety of sulfated flavonoids and accumulated spectral data in order to identify the compounds in . The LC-MS analysis of six crude drug samples revealed the presence of luteolin 7-sulfate, apigenin 7-sulfate, diosmetin 7-sulfate, and diosmetin 3'-sulfate. The samples without luteolin 3'-sulfate featured high apigenin 7-sulfate content. Although the samples were collected from the same locality, their compositions differed depending on the year of collection. Further, they were classified according to three patterns: (1) samples with luteolin 7-sulfate as the main component, (2) samples with apigenin 7-sulfate as the main component, and (3) samples with relatively high diosmetin sulfate content. Luteolin 7-sulfate typically exhibits relatively high erythrocyte aggregation efficiency and fibrinogen aggregation rate. These results demonstrate that the analysis of sulfated flavonoids is beneficial for the quality evaluation of for hemostatic applications.
（菊科）因其含有硫酸化黄酮类化合物而被许多传统药物用作止血剂。在这项研究中，我们从三个不同年份采集的三个省份获得了原料药样本，并使用液相色谱-质谱联用（LC-MS）对其硫酸化黄酮类化合物含量进行了分析，以进行质量评估。由于硫酸化黄酮类化合物不稳定，且难以从提取物中分离出来，因此本研究首先合成了多种硫酸化黄酮类化合物，并积累了光谱数据，以便鉴定 中的化合物。对六种原料药样本的 LC-MS 分析表明，存在木樨草素 7-硫酸盐、芹菜素 7-硫酸盐、香叶木素 7-硫酸盐和香叶木素 3'-硫酸盐。没有木樨草素 3'-硫酸盐的样本中，芹菜素 7-硫酸盐含量较高。尽管这些样本是从同一地点采集的，但它们的成分因采集年份而异。此外，它们根据三种模式进行了分类：（1）以木樨草素 7-硫酸盐为主要成分的样本，（2）以芹菜素 7-硫酸盐为主要成分的样本，以及（3）硫酸化香叶木素含量相对较高的样本。木樨草素 7-硫酸盐通常表现出相对较高的红细胞聚集效率和纤维蛋白原聚集率。这些结果表明，对硫酸化黄酮类化合物的分析有助于止血应用中 质量评估。
