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炮姜叶化学成分研究、斑马鱼毒性评价及肠道止血作用研究

Study on chemical constituents of Folium Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis.

作者信息

Gu Le, Wang Xueyu, Shao Xinting, Ding Yuling, Li Yong

机构信息

School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, China.

出版信息

Saudi Pharm J. 2022 May;30(5):532-543. doi: 10.1016/j.jsps.2022.02.018. Epub 2022 Mar 2.

DOI:10.1016/j.jsps.2022.02.018
PMID:35693441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9177460/
Abstract

Folium Carbonisatum (FAAC) is a traditional medicine widely used in clinic. It has the effect of hemostasis by warming meridians. In order to further explore the chemical composition and biological activity of FAAC, the methanol extract of FAAC was isolated and purified by open column and high- performance liquid chromatography. and the complete structure was characterized by nuclear magnetic resonance (NMR) and LREI-MS for the first time, namely rutin, quercetin and octacosanol respectively. Initially the toxic effect of methanol extract of FAAC on zebrafish was evaluated by observing the phenotypic characteristics, spontaneous twitch times, heart rate, hatching rate, the distance of SV-BA and cardiomyocyte apoptosis of zebrafish. The results showed that FAAC has embryonic development toxicity and cardiotoxicity when it was higher than 62.5 μg/mL. Meanwhile, the hemostatic effect of methanol extract of FAAC was compared with FAA (Folium ) by zebrafish intestinal bleeding model originally. The results showed that the hemostatic effect of the medium and high concentration dose groups (3.0 and 30.0 μg/mL) was enhanced for both FAAC and FAA. This study provided an experimental basis for the clinical application of FAAC.

摘要

炮姜是一种临床广泛应用的传统中药。其具有温经止血的功效。为进一步探究炮姜的化学成分及生物活性，采用常压柱色谱和高效液相色谱对炮姜甲醇提取物进行分离纯化，并首次通过核磁共振（NMR）和电喷雾电离质谱（LREI-MS）对其完整结构进行表征，分别为芦丁、槲皮素和二十八烷醇。首先，通过观察斑马鱼的表型特征、自发抽搐次数、心率、孵化率、体节-肛前距以及心肌细胞凋亡情况，对炮姜甲醇提取物对斑马鱼的毒性作用进行评价。结果表明，炮姜浓度高于62.5μg/mL时对斑马鱼具有胚胎发育毒性和心脏毒性。同时，最初通过斑马鱼肠道出血模型比较了炮姜甲醇提取物与艾叶（FAA）的止血效果。结果表明，炮姜和艾叶的中、高浓度剂量组（3.0和30.0μg/mL）的止血效果均增强。本研究为炮姜的临床应用提供了实验依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/481820dded59/gr10.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/00048020431b/gr3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/ddbfaeeb7b82/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/127b2547b98f/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/c24f09dcae5e/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/481820dded59/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/cb35e5701191/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/0da40eed50c0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/6d64972cc898/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/00048020431b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/a00aae30b1e0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/3db7e9e2d246/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/2b28da61cb60/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/ddbfaeeb7b82/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/127b2547b98f/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/c24f09dcae5e/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e950/9177460/481820dded59/gr10.jpg

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