Meena G G, Stambaugh A M, Ganjalizadeh V, Stott M A, Hawkins A R, Schmidt H
School of Engineering, University of California, Santa Cruz, 1156 High Street, Santa Cruz, California 95064, USA.
Electrical and Computer Engineering Department, Brigham Young University, Provo, Utah 84602, USA.
APL Photonics. 2021 Jun;6(6). doi: 10.1063/5.0049735. Epub 2021 Jun 1.
Nucleic acids and proteins are the two most important target types used in molecular diagnostics. In many instances, simultaneous sensitive and accurate detection of both biomarkers from the same sample would be desirable, but standard detection methods are highly optimized for one type and not cross-compatible. Here, we report the simultaneous multiplexed detection of SARS-CoV-2 RNAs and antigens with single molecule sensitivity. Both analytes are isolated and labeled using a single bead-based solid-phase extraction protocol, followed by fluorescence detection on a multi-channel optofluidic waveguide chip. Direct amplification-free detection of both biomarkers from nasopharyngeal swab samples is demonstrated with single molecule detection sensitivity, opening the door for ultrasensitive dual-target analysis in infectious disease diagnosis, oncology, and other applications.
核酸和蛋白质是分子诊断中使用的两种最重要的靶标类型。在许多情况下，人们希望能同时从同一样本中灵敏且准确地检测这两种生物标志物，但标准检测方法针对一种类型进行了高度优化，且不具有交叉兼容性。在此，我们报告了以单分子灵敏度同时多重检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的RNA和抗原。两种分析物都使用基于单个珠子的固相萃取方案进行分离和标记，然后在多通道光流体波导芯片上进行荧光检测。利用单分子检测灵敏度，证明了可从鼻咽拭子样本中直接对两种生物标志物进行无扩增检测，为传染病诊断、肿瘤学及其他应用中的超灵敏双靶标分析打开了大门。
