Opt Express. 2020 Oct 26;28(22):33019-33027. doi: 10.1364/OE.402311.
Rapid and accurate diagnosis of bacterial infections resistant to multiple antibiotics requires development of new bio-sensors for differentiated detection of multiple targets. This work demonstrates 7x multiplexed detection for antibiotic-resistance bacterial screening on an optofluidic platform. We utilize spectrally multiplexed multi-spot excitation for simultaneous detection of nucleic acid strands corresponding to bacterial targets and resistance genes. This is enabled by multi-mode interference (MMI) waveguides integrated in an optofluidic device. We employ a combinatorial three-color labeling scheme for the nucleic acid assays to scale up their multiplexing capability to seven different nucleic acids, representing three species and four resistance genes.
快速准确地诊断对抗生素具有多重耐药性的细菌感染需要开发新的生物传感器，以实现对多种目标的差异化检测。本工作展示了在光流控平台上进行 7 重抗生素耐药细菌筛选的多重检测。我们利用光谱多重分束激发，同时检测与细菌靶标和耐药基因相对应的核酸链。这是通过集成在光流控器件中的多模干涉（MMI）波导实现的。我们采用组合三颜色标记方案进行核酸检测，以将其多重检测能力扩展到七种不同的核酸，代表三种物种和四种耐药基因。
