Africa Health Research Institute, Nelson R. Mandela School of Medicine, University KwaZulu-Natal, Durban, South Africa.
Human Immunodeficiency Virus (HIV) Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.
Front Cell Infect Microbiol. 2022 May 27;12:908144. doi: 10.3389/fcimb.2022.908144. eCollection 2022.
There is an urgent need for accurate and sensitive diagnostic tools that can overcome the current challenge to distinguish individuals with latent tuberculosis infection (LTBI) from individuals with active tuberculosis (TB). Recent literature has suggested that a group of cytokines may serve as biomarkers of TB disease progression. Using a multiplex ELISA, we quantified 27 circulatory markers present within the unstimulated plasma of individuals in Durban, South Africa who were healthy (n=20), LTBI (n=13), or had active TB (n=30). RT-qPCR was performed to measure gene expression of the cytokines of interest, using RNA isolated from healthy (n=20), LTBI (n=20), or active TB (n=30). We found that at the protein level, IL-1RA, IL-6, and IP-10 were significantly more abundant in participants with active TB (p< 0.05) compared to those with LTBI individuals. IP-10 also showed the strongest association with active TB compared to healthy and LTBI at mRNA level. Our data shows that these proteins may serve as biomarkers of TB at both the protein and gene level.
目前迫切需要准确和敏感的诊断工具，以克服当前的挑战，区分潜伏性结核感染（LTBI）个体和活动性结核病（TB）个体。最近的文献表明，一组细胞因子可以作为结核病疾病进展的生物标志物。我们使用多重 ELISA 定量分析了南非德班健康个体（n=20）、LTBI 个体（n=13）和活动性 TB 个体（n=30）的未刺激血浆中存在的 27 种循环标志物。使用从健康个体（n=20）、LTBI 个体（n=20）或活动性 TB 个体（n=30）中分离的 RNA，通过 RT-qPCR 测量感兴趣的细胞因子的基因表达。我们发现，在蛋白水平上，与 LTBI 个体相比，活动性 TB 个体中 IL-1RA、IL-6 和 IP-10 的含量明显更高（p<0.05）。与健康和 LTBI 相比，IP-10 在 mRNA 水平上与活动性 TB 的相关性最强。我们的数据表明，这些蛋白质可以作为 TB 的蛋白和基因水平的生物标志物。
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