Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma, 371-8511, Japan.
Sci Rep. 2022 Jun 13;12(1):9749. doi: 10.1038/s41598-022-13965-w.
There are conflicting reports regarding the efficacy of cortisol as a stress marker in altitude training due to the influence of the circadian rhythm. This study aimed to verify whether the automated measurement of salivary cortisol concentration via sequential sampling could detect the differences in exercise stress between two altitudes. We enrolled 12 elite female long-distance runners living near sea level. For the first higher-altitude camp, the runners lived at 1800 m and trained at 1700 m for 7 days. For the second lower-altitude camp, they lived at 1550 m and trained at 1300 m for 7 days. Their saliva was sequentially collected on the last 2 days during each camp which involved different intensity exercises in the morning and afternoon. The salivary cortisol concentrations were measured using electrochemiluminescence immunoassay. Before dinner, the basal salivary cortisol concentrations were significantly higher in the higher-altitude camp. The rate of change in the salivary cortisol concentration during the morning exercise was significantly higher in the higher-altitude camp than in lower-altitude camp (p = 0.028) despite the same exercise programs and intensities. Salivary cortisol level measurements during the athletes' circadian rhythms could detect the differences in acclimatization and exercise stress between two altitudes.
由于昼夜节律的影响，关于皮质醇作为高原训练应激标志物的功效存在相互矛盾的报告。本研究旨在验证通过序贯采样自动测量唾液皮质醇浓度是否可以检测出两种海拔高度之间运动应激的差异。我们招募了 12 名居住在海平面附近的精英女性长跑运动员。对于第一个高海拔营地，运动员们在 1800 米处居住，并在 1700 米处训练 7 天。对于第二个低海拔营地，他们在 1550 米处居住，并在 1300 米处训练 7 天。在每个营地的最后两天，他们的唾液被序贯收集，涉及上午和下午不同强度的运动。使用电化学发光免疫分析法测量唾液皮质醇浓度。晚餐前，高海拔营地的基础唾液皮质醇浓度明显更高。尽管运动计划和强度相同，但高海拔营地的早晨运动期间唾液皮质醇浓度的变化率明显高于低海拔营地（p=0.028）。在运动员的昼夜节律期间进行唾液皮质醇水平测量，可以检测出两种海拔高度之间的适应和运动应激的差异。
