State Key Laboratory Base of Eco-chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
State Key Laboratory Base of Eco-chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; Institute of High Performance Polymers, Qingdao University of Science and Technology, Qingdao 266042, China.
Carbohydr Polym. 2022 Sep 1;291:119522. doi: 10.1016/j.carbpol.2022.119522. Epub 2022 Apr 27.
This work aimed to develop a novel chitosan based metal-organic polyhedrons (MOPs)/enzyme hybrid hydrogel with superior antimicrobial properties in wound healing treatment. Hybrid hydrogel was prepared by crosslinking glucose oxidase (GOx), vanadium metal-organic polyhedrons (VMOP-2) and chitosan using glutaraldehyde as crosslinker. The formed GVCS hydrogel was characterized by using various techniques, including FTIR, SEM, XPS, TGA and EDX. Data show that GVCS hydrogel was successfully obtained with uniform distribution of GOx and VMOP-2 in the hydrogel structure. Antibacterial tests show that GVCS hydrogel exhibits better bactericidal effect on both gram-negative bacteria (S. aureus) and gram-positive bacteria (E. coli) compared to other hydrogel samples because of its hydroxyl radicals generation capacity in the presence of glucose. MTT assay shows that the hydrogel presents good cell compatibility. In vivo experiments using an infected wound model indicate that GVCS hydrogel can effectively facilitate wound healing. Therefore, chitosan based MOPs/enzyme hybrid hydrogel could be most promising for antibacterial therapy in clinical applications.
本工作旨在开发一种新型壳聚糖基金属有机多面体（MOPs）/酶杂化水凝胶，用于伤口愈合治疗，具有优异的抗菌性能。杂化水凝胶是通过交联葡萄糖氧化酶（GOx）、钒金属有机多面体（VMOP-2）和壳聚糖，使用戊二醛作为交联剂制备的。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、X 射线光电子能谱（XPS）、热重分析（TGA）和能谱（EDX）等技术对形成的 GVCS 水凝胶进行了表征。数据表明，GOx 和 VMOP-2 均匀分布在水凝胶结构中，成功制备了 GVCS 水凝胶。抗菌试验表明，由于葡萄糖存在时能够产生羟基自由基，GVCS 水凝胶对革兰氏阴性菌（金黄色葡萄球菌）和革兰氏阳性菌（大肠杆菌）均具有更好的杀菌效果，优于其他水凝胶样品。MTT 试验表明，该水凝胶具有良好的细胞相容性。利用感染性伤口模型进行的体内实验表明，GVCS 水凝胶可有效促进伤口愈合。因此，基于壳聚糖的 MOPs/酶杂化水凝胶有望在临床应用中成为一种很有前途的抗菌治疗方法。
