College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
Carbohydr Polym. 2022 Sep 1;291:119580. doi: 10.1016/j.carbpol.2022.119580. Epub 2022 May 7.
A polysaccharides-based delivery system was designed to encapsulate and control the release of peanut peptide (PP). The PP-loaded polyelectrolyte complex (TMC-PP-SA) was fabricated based on the electrostatic self-assembly between n-trimethy chitosan (TMC) and sodium alginate (SA). The complex exhibited uniform spherical morphology, satisfactory stability and high encapsulation efficiency. In vitro release behavior indicated that TMC-PP-SA polyelectrolyte complex could inhibit the release of PP at simulated gastric medium and enhance the release of PP at simulated intestinal medium. Moreover, the antioxidant activity of PP after encapsulation was significantly improved compared with that of directly digested PP. Ex vivo intestinal permeation study confirmed that about 41.76 ± 1.43% PP in TMC-PP-SA could be absorbed in the intestinal. The cytotoxicity measurement indicated that the fabricated TMC-PP-SA polyelectrolyte complex was biocompatible and nontoxic. Therefore, these results indicated that the polysaccharides-based delivery system had great potential in protecting active peptides from degradation and facilitating their absorption.
设计了一种基于多糖的递药系统来包裹并控制花生肽（PP）的释放。通过静电自组装将 n-三甲基壳聚糖（TMC）和海藻酸钠（SA）制备成载肽的聚电解质复合物（TMC-PP-SA）。该复合物具有均匀的球形形态、良好的稳定性和较高的包封效率。体外释放行为表明，TMC-PP-SA 聚电解质复合物在模拟胃液中能抑制 PP 的释放，而在模拟肠液中能增强 PP 的释放。此外，与直接消化的 PP 相比，包封后的 PP 的抗氧化活性显著提高。离体肠渗透研究证实，TMC-PP-SA 中的约 41.76±1.43%的 PP 可在肠道中被吸收。细胞毒性测量表明，所制备的 TMC-PP-SA 聚电解质复合物具有良好的生物相容性和低毒性。因此，这些结果表明基于多糖的递药系统在保护活性肽免受降解和促进其吸收方面具有巨大的潜力。
