Cohn D A, Schiffman G
Infect Immun. 1987 Jun;55(6):1375-80. doi: 10.1128/iai.55.6.1375-1380.1987.
Antibody responses to two structurally different pneumococcal polysaccharides, type 3 (SIII) and type 14 (SXIV), were examined in intact and splenectomized (Sx) A/J mice to determine whether the role of the spleen in immune responses to these antigens varies with respect to the dosage, the antigenic structure, or the interval between immunization and assay. Antibody responses to SIII and SXIV, measured over a 4-week period by radioimmunoassay, differed in antigenic load requirements, kinetics, and extent of dependence upon the spleen. Intact mice given 50 or 100 ng of SIII produced peak antibody responses on day 5, which tapered off by days 14 and 21. Intact mice given SXIV required doses 100 times greater than those of SIII to stimulate high levels of antibody response; antibody responses increased on day 5 and remained elevated through day 28. In Sx mice given 50 or 100 ng of SIII, the peak antibody response on day 5 was obliterated, but extrasplenic sources produced low levels of antibody which peaked by day 14. In Sx mice given SXIV, all anti-SXIV responses were abrogated regardless of the dose or day of assay. Differences between the anti-SIII and anti-SXIV responses in dependence upon the spleen were probably due to structural differences between the two antigens and to the localization of each to different sites in the reticuloendothelial system. These results attest to the importance of the spleen in antibacterial resistance. They show that, even in the presence of extrasplenic antibody synthesis, the spleen is required for early antibody production, the timing of which is critical for the effective clearance of bacteria.
在完整和脾切除（Sx）的A/J小鼠中检测了对两种结构不同的肺炎球菌多糖3型（SIII）和14型（SXIV）的抗体反应，以确定脾脏在针对这些抗原的免疫反应中的作用是否因剂量、抗原结构或免疫与检测之间的间隔而有所不同。通过放射免疫测定在4周内测量的对SIII和SXIV的抗体反应在抗原负荷需求、动力学以及对脾脏的依赖程度方面存在差异。给予50或100 ng SIII的完整小鼠在第5天产生抗体反应峰值，到第14天和21天逐渐下降。给予SXIV的完整小鼠需要比SIII大100倍的剂量来刺激高水平的抗体反应；抗体反应在第5天增加并持续升高至第28天。给予50或100 ng SIII的Sx小鼠在第5天的抗体反应峰值消失，但脾外来源产生低水平的抗体，在第14天达到峰值。给予SXIV的Sx小鼠，无论剂量或检测日期如何，所有抗SXIV反应均被消除。抗SIII和抗SXIV反应在对脾脏的依赖上的差异可能是由于两种抗原之间的结构差异以及它们在网状内皮系统中各自定位到不同部位所致。这些结果证明了脾脏在抗菌抗性中的重要性。它们表明，即使存在脾外抗体合成，脾脏对于早期抗体产生也是必需的，而早期抗体产生的时间对于有效清除细菌至关重要。
