5.8GHz 微波对大鼠睾丸结构和功能的影响。

Effects of 5.8 GHz Microwaves on Testicular Structure and Function in Rats.

机构信息

Department of Radiation Protection Medicine, School of Military Preventive Medicine, Air Force Medical University, Xi'an, China.

Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an, China.

出版信息

Biomed Res Int. 2022 Jun 6;2022:5182172. doi: 10.1155/2022/5182172. eCollection 2022.

OBJECTIVE

To investigate the effects of exposure to 5.8 GHz microwaves on testicular structure and function of male adult rats.

METHODS

After 30 days of exposure, we evaluated sperm quality by determining sperm concentration and quantifying the number of abnormal sperm. Testicular morphology was investigated by hematoxylin-eosin (HE) staining. The levels of testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH), glial cell line-derived neurotrophic factor (GDNF), stem cell factor (SCF), and transferrin (TRF) were determined by enzyme-linked immunosorbent assays (ELISAs). We also used western blotting to determine the levels of GDNF and SCF and apoptosis-related protein (caspase-3) in the testis.

RESULTS

Compared with the sham group, there were no significant differences in terms of sperm count, sperm abnormality, and the levels of T, FSH, LH, GDNF, SCF, and caspase-3 in the microwave group.

CONCLUSION

Under the experimental conditions, 5.8 GHz microwave exposure has no obvious effect on testicular structure and function of rats.

目的

研究 5.8GHz 微波辐射对成年雄性大鼠睾丸结构和功能的影响。

方法

暴露 30 天后，通过测定精子浓度和精子畸形数来评估精子质量。通过苏木精-伊红（HE）染色观察睾丸形态。采用酶联免疫吸附试验（ELISA）测定睾酮（T）、卵泡刺激素（FSH）、黄体生成素（LH）、胶质细胞源性神经营养因子（GDNF）、干细胞因子（SCF）和转铁蛋白（TRF）的水平。还使用 Western blot 测定睾丸中 GDNF 和 SCF 以及凋亡相关蛋白（caspase-3）的水平。