ICMR-National Institute of Malaria Research, New Delhi, India.
J Vector Borne Dis. 2022 Jan-Mar;59(1):52-56. doi: 10.4103/0972-9062.331414.
BACKGROUND & OBJECTIVES: Attractive toxic sugar baits (ATSB) is a novel tool which employs mosquito sugar feeding behaviour to kill them. The potential of ATSB against mosquito vectors has been demonstrated in limited scope around the world including Israel, some of the African countries and USA. But their efficacy against mosquito vectors of India is yet to be ascertained. Therefore, current study was planned to evaluate the efficacy of TSB (without attractant) against two important malaria vectors Anopheles culicifacies and An. stephensi along with major dengue vector Aedes aegypti.
TSB solution was prepared by dissolving different concentrations of boric acid in glucose and tested against each of the mosquito species. Another experiment was done by spraying this boric acid sugar solution on to Calendula officinalis plant. It served as a sole source of mosquito feed and mortality of mosquitoes was counted after 24 hours.
The TSB resulted in 100% mortality of Ae. aegypti and An. stephensi at 4% concentration of boric acid while in An. culicifacies 100% mortality was achieved at 3% concentration TSB solution. TSB solution with 2% boric acid, resulted in 99.1% mortality in An. culicifacies while ~95 % mortality of An. stephensi and Ae. aegypti. When TSB solution with 2% boric acid was sprayed on plant, ~89-94% mortality was observed in both An. stephensi and An. culicifacies.
INTERPRETATION & CONCLUSION: Based on the results, boric acid based toxic sugar bait solution could be a promising tool for vector control. Further studies are needed to find out its toxicity of TSB against non-target organisms and residual efficacy in field trials in different ecotypes.
有吸引力的毒性糖诱饵（ATSB）是一种新型工具，利用蚊子的取食行为来杀死它们。这种工具在全球范围内的一些地方，包括以色列、一些非洲国家和美国，已经在有限的范围内展示了其对蚊子媒介的潜力。但是，它们对印度蚊子媒介的效果还需要确定。因此，目前的研究计划评估 TSB（不含引诱剂）对两种重要的疟疾媒介按蚊和斯蒂芬斯按蚊以及主要的登革热媒介埃及伊蚊的功效。
通过在葡萄糖中溶解不同浓度的硼酸来制备 TSB 溶液，并对每种蚊子进行测试。另一个实验是将这种硼酸糖溶液喷洒在金盏花植物上。它是蚊子唯一的食物来源，24 小时后统计蚊子的死亡率。
TSB 溶液在 4%的硼酸浓度下导致埃及伊蚊和斯蒂芬斯按蚊 100%死亡，而在库蚊中，100%的死亡率是在 3%的 TSB 溶液浓度下实现的。硼酸浓度为 2%的 TSB 溶液导致库蚊的死亡率为 99.1%，而斯蒂芬斯按蚊和埃及伊蚊的死亡率约为 95%。当 2%硼酸的 TSB 溶液喷洒在植物上时，斯蒂芬斯按蚊和库蚊的死亡率分别约为 89-94%。
根据结果，硼酸基毒性糖诱饵溶液可能是一种有前途的控制媒介的工具。需要进一步研究以确定 TSB 对非目标生物的毒性及其在不同生态型中的田间试验中的残留功效。
