Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori 036-8561, Japan.
Department of Materials Science and Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
Bioresour Technol. 2022 Sep;359:127479. doi: 10.1016/j.biortech.2022.127479. Epub 2022 Jun 14.
cis,cis-Muconate (ccMA) is a promising platform for use in synthesizing various polymers. A glucose-free ccMA production using Pseudomonas sp. NGC7 from hardwood lignin-derived aromatic compounds was previously reported. In that system, syringyl nucleus compounds were essential for growth. Here, it is shown that NGC7 is available for glucose-free ccMA production even from a mixture of lignin-derived aromatics that does not contain syringyl nucleus compounds. By introducing a gene set for the protocatechuate (PCA)-shunt consisting of PCA 3,4-dioxygenase and PCA decarboxylase into an NGC7-derived strain deficient in PCA 3,4-dioxygenase and ccMA cycloisomerase, it was succeeded in constructing a ccMA-producing strain that grows on a lignin-derived aromatics mixture containing no syringyl nucleus compounds. Finally, it is demonstrated that the engineered strain produced ccMA from sugar cane bagasse alkaline extract in 18.7 mol%. NGC7 is thus shown to be a promising microbial chassis for biochemicals production from lignin-derived aromatics.
顺,顺-黏康酸（ccMA）是一种很有前途的平台，可用于合成各种聚合物。先前有报道称，从硬木木质素衍生的芳香族化合物中使用假单胞菌 NGC7 生产无葡萄糖 ccMA。在该系统中，愈创木基核化合物对生长是必需的。在这里，结果表明，即使在不含有愈创木基核化合物的木质素衍生芳烃混合物中，NGC7 也可用于无葡萄糖 ccMA 的生产。通过将包含原儿茶酸（PCA）-支路的基因集（由 PCA 3,4-加氧酶和 PCA 脱羧酶组成）引入到 PCA 3,4-加氧酶和 ccMA 环化异构酶缺陷的 NGC7 衍生菌株中，成功构建了一株能够在含有无愈创木基核化合物的木质素衍生芳烃混合物上生长的 ccMA 生产菌株。最后，证明了该工程菌株能够从甘蔗渣碱性提取物中以 18.7mol%的产率生产 ccMA。因此，NGC7 被证明是一种很有前途的从木质素衍生芳烃生产生物化学物质的微生物底盘。
