Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, Jilin, China; College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, Jilin, China.
Int J Biol Macromol. 2022 Aug 1;214:312-323. doi: 10.1016/j.ijbiomac.2022.06.071. Epub 2022 Jun 15.
Type 2 diabetes mellitus is a global disease that endangers human health, and the need for the development of nontoxic treatment candidates is urgent. In the present work, one homogeneous polysaccharide from Inonotus obliquus (IN) was isolated, and the protective effect and mechanism of IN on type 2 diabetes mellitus were investigated from the aspects of the intestinal barrier. IN mainly consisted of 9 monosaccharides with a Mw of 373 kDa. IN attenuated body weight loss, alleviated pathological damage, and suppressed the production of proinflammatory cytokines. Additionally, IN repaired the intestinal barrier by upregulating the expression of Ki-67, ZO-1 and MUC2. Furthermore, the abundance of Firmicutes was significantly increased with IN treatment, while the levels of Bacteroidetes were significantly inhibited. In conclusion, IN protected against type 2 diabetes mellitus by ameliorating intestinal barrier dysfunction and might serve as a novel drug candidate for type 2 diabetes mellitus.
2 型糖尿病是一种危害人类健康的全球性疾病，迫切需要开发无毒的治疗候选物。本工作从肠道屏障方面分离得到一种桦褐孔菌（Inonotus obliquus，IN）均一多糖，并研究了 IN 对 2 型糖尿病的保护作用及其机制。IN 主要由 9 种单糖组成，Mw 为 373 kDa。IN 可减轻体重减轻，减轻病理损伤，并抑制促炎细胞因子的产生。此外，IN 通过上调 Ki-67、ZO-1 和 MUC2 的表达来修复肠道屏障。此外，IN 治疗后厚壁菌门的丰度显著增加，而拟杆菌门的水平则显著受到抑制。总之，IN 通过改善肠道屏障功能来预防 2 型糖尿病，可能成为 2 型糖尿病的新型药物候选物。
