Zhang Cai, Li Chenxu, Zhao Pengli, Shao Qi, Ma Yanbo, Bai Dongying, Liao Chengshui, He Lei, Huang Shucheng, Wang Xueying
Henan International Joint Laboratory of Animal Welfare and Health Breeding, Henan University of Science and Technology, Luoyang, P. R. China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, P. R. China.
Anim Biotechnol. 2023 Dec;34(7):2273-2284. doi: 10.1080/10495398.2022.2086878. Epub 2022 Jun 17.
The aim of this study was to evaluate the effects of dietary polysaccharide (GCP) supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets. One hundred and twenty 28-day-old weaned piglets were randomly assigned into five groups (four replicates per group) and fed a basal diet with GCP at 0, 500, 1000, 2000 and 4000 mg/kg for four weeks, respectively. Results showed that 1000 mg/kg GCP improved piglets' ADG and ADFI and reduced FCR ( < .05). Thus, the 0 and 1000 mg/kg GCP dose were selected for subsequent experiments. We found that 1000 mg/GCP increased SOD and T-AOC and decreased MDA in the jejunal mucosa ( < .05). Dietary 1000 mg/kg GCP also resulted in high levels of sIgA, IL-10 and TGF-β, whereas IL-2 dropped dramatically ( < .05). The relative expression levels of , , , , , , , and increased in the jejunal mucosa, whereas decreased ( < .05). 1000 mg/kg GCP treatment altered the diversity and community composition of cecal microbiota in pigs, with increasing relative abundance of Bacteroidota and at phylum and genus levels ( < .05), respectively. The results suggested that dietary 1000 mg/kg GCP could improve growth performance and intestinal health of weaned piglets.
本研究旨在评估日粮中添加多糖（GCP）对断奶仔猪生长性能、肠道抗氧化剂、免疫力和微生物群的影响。将120头28日龄断奶仔猪随机分为五组（每组四个重复），分别饲喂添加0、500、1000、2000和4000 mg/kg GCP的基础日粮，持续四周。结果表明，1000 mg/kg GCP提高了仔猪的平均日增重（ADG）和平均日采食量（ADFI），并降低了料重比（FCR）（P<0.05）。因此，选择0和1000 mg/kg GCP剂量进行后续实验。我们发现，1000 mg/kg GCP增加了空肠黏膜中的超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC），并降低了丙二醛（MDA）水平（P<0.05）。日粮中添加1000 mg/kg GCP还导致分泌型免疫球蛋白A（sIgA）、白细胞介素-10（IL-10）和转化生长因子-β（TGF-β）水平升高，而白细胞介素-2（IL-2）显著下降（P<0.05）。空肠黏膜中Occludin、Claudin-1、ZO-1、JAM-1、MUC2、TFF3、IL-10、TGF-β和IgA的相对表达水平升高，而IL-2降低（P<0.05）。1000 mg/kg GCP处理改变了猪盲肠微生物群的多样性和群落组成，在门和属水平上分别增加了拟杆菌门（Bacteroidota）和普雷沃氏菌属（Prevotella）的相对丰度（P<0.05）。结果表明，日粮中添加1000 mg/kg GCP可改善断奶仔猪的生长性能和肠道健康。
