Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Talanta. 2022 Nov 1;249:123675. doi: 10.1016/j.talanta.2022.123675. Epub 2022 Jun 13.
Development of new hot spots for surface enhanced Raman scattering (SERS) technique is of great significance recently. Herein, we developed a single Au nanowire (NW)-based nanosensor for adenosine triphosphate (ATP) sensing by using in-situ SERS technique. Single Au NWs, fabricated by laser-assisted pulling method and hydrofluoric acid (HF) etching process, were linked with single-stranded HS-terminated DNA. After that, gold-silver bimetallic nanoparticles (Au/Ag NPs), attached with thiol-containing Raman dyes and ATP aptamer, were immobilized on DNA-modified single AuNW due to the designed affinity between ATP aptamer and single-stranded DNA. This single AuNW-based device exhibited strong SERS signals. In the presence of adenosine triphosphate (ATP), due to the strong specific affinity between the aptamer and the target, the Au/Ag NPs will be separated from the AuNW, resulting in the obvious decrease of the Raman signals, which can be used for ATP sensing with high sensitivity, selectivity and stability. This nanosensor can be used as an ideal platform for real applications, especially at some confined-space samples, such as trace detection, single cell and in vivo analysis.
最近，开发新的表面增强拉曼散射（SERS）技术热点具有重要意义。在此，我们通过原位 SERS 技术开发了一种基于单个金纳米线（NW）的用于三磷酸腺苷（ATP）传感的纳米传感器。通过激光辅助拉伸法和氢氟酸（HF）刻蚀工艺制备的单个 Au NW 与巯基 HS 封端的 DNA 相连。之后，由于 ATP 适体与单链 DNA 之间的设计亲和力，附着有含硫醇的拉曼染料和 ATP 适体的金银双金属纳米颗粒（Au/Ag NPs）被固定在 DNA 修饰的单个 AuNW 上。这种基于单个 AuNW 的器件表现出很强的 SERS 信号。在存在三磷酸腺苷（ATP）的情况下，由于适体与靶物之间的强特异性亲和力，Au/Ag NPs 将从 AuNW 上分离出来，导致拉曼信号明显下降，可用于高灵敏度、选择性和稳定性的 ATP 传感。该纳米传感器可用作实际应用的理想平台，特别是在一些受限空间样品中，如痕量检测、单细胞和体内分析。
