Alalwani Aisha D, Hummdi Laila A, Qahl Safa H
Department of Biology, Science College, University of Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2022 Jul;29(7):103333. doi: 10.1016/j.sjbs.2022.103333. Epub 2022 Jun 1.
The study aimed to evaluate the impact of mixture and leaf nano extracts, and liraglutide, on liver tissue and serum lipids in type 2 diabetic male albino rat model. Forty rats were divided equally into 4 groups were used. Group 1 was the non-diabetic control group. The animals in Groups 2-4 was injected intraperitoneally with a single dose of 60 mg/kg b.w. Streptozotocin to induce a diabetic rat model. Group 2 served as a positive control for diabetes. 0.02 mg/kg b.w./day of Liraglutide gave to groups 3 and 4 and 4.8 ng/ml × 10 b.w./day of a mixture of the nano extracts, respectively. Eight weeks after treatment, the animals were sacrificed. Blood was collected for glucose analysis and serum low-density lipoprotein, high-density lipoprotein, total cholesterol, and triglycerides analysis, and the livers processed for histopathological examination. The elevated lipid profiles and blood glucose levels in diabetic group (Group 2) were significantly reduced (p < 0.001) following the administration of liraglutide and nano extracts in Groups 3 and 4. Progressive fatty acid changes were found in the liver sections, indicated by the deposition of various sizes of lipid droplets in most liver lobules, along with patchy hepatocyte necrosis. These pathological changes were ameliorated in the liraglutide- and nano-extract-treated rats. Treatment with the nano extracts resulted in significant power assays associated with recovery of hepatic histology and functional alterations, compared to liraglutide treatment.
该研究旨在评估混合纳米提取物和利拉鲁肽对2型糖尿病雄性白化大鼠模型肝脏组织和血脂的影响。将40只大鼠平均分为4组。第1组为非糖尿病对照组。第2 - 4组动物腹腔注射单剂量60 mg/kg体重的链脲佐菌素以诱导糖尿病大鼠模型。第2组作为糖尿病阳性对照组。分别给予第3组和第4组0.02 mg/kg体重/天的利拉鲁肽和4.8 ng/ml×10体重/天的纳米提取物混合物。治疗8周后，处死动物。采集血液进行血糖分析以及血清低密度脂蛋白、高密度脂蛋白、总胆固醇和甘油三酯分析，并对肝脏进行组织病理学检查。在第3组和第4组给予利拉鲁肽和纳米提取物后，糖尿病组（第2组）升高的血脂水平和血糖水平显著降低（p < 0.001）。在肝脏切片中发现进行性脂肪酸变化，表现为大多数肝小叶中出现大小不一的脂滴沉积，伴有散在的肝细胞坏死。这些病理变化在接受利拉鲁肽和纳米提取物治疗的大鼠中得到改善。与利拉鲁肽治疗相比，纳米提取物治疗导致与肝脏组织学恢复和功能改变相关的显著功效分析。
