复发性尿路感染女性与非复发性尿路感染女性之间的尿液微生物群组成差异
Differential Urinary Microbiota Composition Between Women With and Without Recurrent Urinary Tract Infection.
作者信息
Huang Lei, Li Xiangyan, Zheng Bo, Li Pengtao, Wei Dali, Huang Chenwei, Sun Liying, Li Haixia
机构信息
Department of Clinical Laboratory, Peking University First Hospital, Beijing, China.
Department of Anti-infection, Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.
出版信息
Front Microbiol. 2022 May 26;13:888681. doi: 10.3389/fmicb.2022.888681. eCollection 2022.
BACKGROUND
Recurrent urinary tract infection (RUTI) is common and burdensome in women. Due to the low concentration or slow-growing of uropathogens in RUTI, standard urine cultures (SUCs) are often negative. Next-generation sequencing (NGS) of bacterial 16S rRNA gene is more sensitive and could be used to reveal the differential microbiota between patients with RUTI and asymptomatic controls.
METHODS
Women (aged ≥ 18 years) with clinically diagnosed RUTI with negative SUC and age-matched women asymptomatic controls with normal urinalysis were enrolled. Their midstream voided urine specimens were collected and processed for NGS (Illumina MiSeq) targeting the bacterial 16S rRNA gene V3-V4 region. The dataset was clustered into operational taxonomic units (OTUs) using QIIME. Taxonomic analysis, alpha diversity, beta diversity, multivariate statistical analysis, and linear discriminant analysis effect size (LEfSe) for differential analysis were performed and compared between patients with RUTI and asymptomatic controls.
RESULTS
A total of 90 patients with RUTI and 62 asymptomatic controls were enrolled in this study. Among them, 74.4% (67/90) and 71.0% (44/62) were successfully amplified and sequenced their bacterial 16S rRNA gene. In the alpha diversity analysis, the chao1 index and observed species index were significantly lower in the RUTI group than in the control group ( = 0.015 and 0.028, respectively). In the beta diversity analysis, there was a significant difference between the 2 groups [Analysis of similarities (ANOSIM), = 0.209, = 0.001]. The relative abundance of 36 bacterial taxa was significantly higher, and another 24 kinds of bacteria were significantly lower in the RUTI group compared with the control group [LEfSe analysis, < 0.05, linear discriminative analysis (LDA) score > 3], suggesting that , , , and may play an important role in RUTI.
CONCLUSION
The urinary microbiota of women with clinically diagnosed RUTI were significantly different from age-matched asymptomatic controls.
背景
复发性尿路感染（RUTI）在女性中很常见且负担较重。由于RUTI中尿路病原体浓度低或生长缓慢，标准尿培养（SUC）往往呈阴性。细菌16S rRNA基因的下一代测序（NGS）更敏感，可用于揭示RUTI患者与无症状对照之间的微生物群差异。
方法
纳入临床诊断为RUTI且SUC阴性的年龄≥18岁女性，以及年龄匹配、尿常规正常的无症状女性对照。收集她们的中段尿标本，针对细菌16S rRNA基因V3-V4区域进行处理后用于NGS（Illumina MiSeq）。使用QIIME将数据集聚类为可操作分类单元（OTU）。进行分类分析、α多样性、β多样性、多变量统计分析以及用于差异分析的线性判别分析效应大小（LEfSe），并在RUTI患者和无症状对照之间进行比较。
结果
本研究共纳入90例RUTI患者和62例无症状对照。其中，分别有74.4%（67/90）和71.0%（44/62）成功扩增并测序了细菌16S rRNA基因。在α多样性分析中，RUTI组的chao1指数和观测物种指数显著低于对照组（分别为P = 0.015和0.028）。在β多样性分析中，两组之间存在显著差异[相似性分析（ANOSIM），R = 0.209，P = 0.001]。与对照组相比，RUTI组中36种细菌分类群的相对丰度显著更高，另外24种细菌显著更低[LEfSe分析，P < 0.05，线性判别分析（LDA）得分>3]，提示[具体细菌名称未给出]可能在RUTI中起重要作用。
结论
临床诊断为RUTI的女性的尿微生物群与年龄匹配的无症状对照有显著差异。
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