Galdiero F, Benedetto N, Quarto N, Romano C
Infect Immun. 1978 Aug;21(2):349-53. doi: 10.1128/iai.21.2.349-353.1978.
The cellular response in the course of experimental infection with Salmonella typhimurium was studied in mice. T cells were detected by the presence of theta-antigen, B cells by the binding of fluorescent immunoglobulins, and cells with receptors by labeled Salmonella binding. Lymphocytes were from spleen and lymph nodes. Results have been divided into three groups: group A, including mice with slight symptomatology; group B, including those with serious infection symptomatology; and group C, including mice that died in the course of the experiment. In spleen and lymph nodes of group A mice, an increase in the percentage of T and B lymphocytes was observed. This increase reached a peak 10 days after experimental infection. In lymph nodes, the B-cell percentage was equal to the percentage of T cells, whereas in spleen lymphocytes the B-cell percentage was higher. In spleens of group B mice we observed the same response as in mice of group A, whereas in lymph nodes there was a low response of T and B lymphocytes. In group C mice, there was no significant response of T and B lymphocytes in either spleen or lymph nodes. In B lymphocytes prepared from spleens of surviving mice, a small number of Salmonella receptors was detected: 200 bacterial cells per 10(9) lymphocytes.
对感染鼠伤寒沙门氏菌的小鼠在实验感染过程中的细胞反应进行了研究。通过θ抗原的存在检测T细胞，通过荧光免疫球蛋白的结合检测B细胞，通过标记的沙门氏菌结合检测具有受体的细胞。淋巴细胞来自脾脏和淋巴结。结果分为三组：A组，包括症状轻微的小鼠；B组，包括有严重感染症状的小鼠；C组，包括在实验过程中死亡的小鼠。在A组小鼠的脾脏和淋巴结中，观察到T淋巴细胞和B淋巴细胞的百分比增加。这种增加在实验感染后10天达到峰值。在淋巴结中，B细胞百分比与T细胞百分比相等，而在脾脏淋巴细胞中B细胞百分比更高。在B组小鼠的脾脏中，我们观察到与A组小鼠相同的反应，而在淋巴结中T淋巴细胞和B淋巴细胞的反应较低。在C组小鼠中，脾脏和淋巴结中的T淋巴细胞和B淋巴细胞均无明显反应。在从存活小鼠脾脏制备的B淋巴细胞中，检测到少量沙门氏菌受体：每10⁹个淋巴细胞中有200个细菌细胞。
