College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, China.
Food Inspection Monitoring Center of Zhuanghe, Dalian, Liaoning Province 116400, China.
J Agric Food Chem. 2022 Jul 6;70(26):8032-8042. doi: 10.1021/acs.jafc.2c02742. Epub 2022 Jun 21.
anthocyanins (AMAs), as natural plant extracts, can control pathogens and are attracting increasing attention. In this study, a tandem mass tag (TMT) quantitative proteomics method combined with multiple reaction monitoring (MRM) was used to explore the antibacterial mechanism of AMAs against at the protein level. The results showed that 1739 proteins were identified in treated with AMAs, of which 628 were altered, including 262 downregulated proteins and 366 upregulated proteins. Bioinformatics analysis showed that these differentially expressed proteins have different molecular functions and participate in different molecular pathways. AMAs can affect protein biosynthesis, DNA replication and repair, oxidative stress response, peptidoglycan biosynthesis, and homeostasis. These pathways induce morphological changes and cell death. The results of this study help understand the molecular mechanism of the inhibitory effect of AMAs on food-borne pathogens and provide a reference for further development of plant-derived antimicrobial agents.
花色苷（AMAs）作为天然植物提取物，可以控制病原体，因此越来越受到关注。在本研究中，采用串联质量标签（TMT）定量蛋白质组学方法结合多重反应监测（MRM）技术，从蛋白质水平探索 AMAs 对 的抗菌机制。结果表明，用 AMAs 处理 后共鉴定到 1739 种蛋白质，其中 628 种蛋白质发生改变，包括 262 种下调蛋白和 366 种上调蛋白。生物信息学分析表明，这些差异表达蛋白具有不同的分子功能，参与不同的分子途径。AMAs 可影响 蛋白的生物合成、DNA 复制和修复、氧化应激反应、肽聚糖生物合成和内稳态。这些途径会诱导形态变化和细胞死亡。本研究结果有助于理解 AMAs 抑制食源性病原体的分子机制，并为进一步开发植物源抗菌剂提供参考。
