School of Life Science, Liaoning University, Chongshan Middle Road 66, Huanggu District, Shenyang, 110036, P. R. China.
Liaoning Academy of Forestry, Yalujiang Street 12, Huanggu District, Shenyang, 110032, P. R. China.
Chem Biodivers. 2020 Nov;17(11):e2000654. doi: 10.1002/cbdv.202000654. Epub 2020 Nov 6.
Colorectal cancer (CRC) is one of the most common malignant tumors in the world. In this study, the Caco-2 in vitro cell model was used to study the effect and mechanism of Aronia melanocarpa (Michx.) Elliott anthocyanins (AMA) on colon cancer. The experimental results showed that the binding energy of anthocyanins on β-catenin was in the range of -5.92 to 4.95 kcal/mol, with good low energy parameters and binding positions. AMA can inhibit cell proliferation and cause cell cycle arrest. RT-PCR and Western blot results showed that AMA can reduce cytoplasmic β-catenin and inhibit the expression of related proteins in Wnt/β-catenin signaling pathway. This study revealed the AMA inhibitory effect and mechanism of malignant biological behavior of Caco-2 cells, in order to provide theoretical basis for the prevention and treatment of colon cancer by Aronia melanocarpa (Michx.) Elliott.
结直肠癌（CRC）是世界上最常见的恶性肿瘤之一。本研究采用体外 Caco-2 细胞模型研究了杨梅（Michx.）Elliott 花色苷（AMA）对结肠癌的作用及机制。实验结果表明，花色苷与 β-连环蛋白的结合能在-5.92 到 4.95 kcal/mol 范围内，具有良好的低能量参数和结合位置。AMA 可以抑制细胞增殖并引起细胞周期停滞。RT-PCR 和 Western blot 结果表明，AMA 可以降低细胞质 β-连环蛋白并抑制 Wnt/β-连环蛋白信号通路相关蛋白的表达。本研究揭示了 AMA 抑制 Caco-2 细胞恶性生物学行为的作用及机制，以期为杨梅预防和治疗结肠癌提供理论依据。
