Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP, Av. do Café, s/n. Bloco S - Sala 41, Ribeirão Preto, SP, 14040-903, Brazil.
Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA.
Sci Rep. 2022 Jun 22;12(1):10555. doi: 10.1038/s41598-022-14492-4.
Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112 S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016. Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants, were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71). Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux pump, were found in all the strains studied. The strains from Brazil carried other resistance genes, such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not found in strains from other countries and were allocated into two distinct clades with strains of human and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin circulating in Brazil for more than 30 years.
都柏林沙门氏菌是一种与牛相关的血清型，偶尔会导致人类患病。本研究对从巴西和其他国家分离的都柏林沙门氏菌菌株进行了分析，以确定它们的系统发育关系、基因、质粒、与毒力和抗菌药物耐药性相关的基因组区域的存在情况，采用 WGS 分析。Illumina 用于对 1983 年至 2016 年间从巴西的人类（n=82）和动物（n=30）中分离的 112 株都柏林沙门氏菌进行基因组测序。此外，还对来自其他国家的 87 株菌株进行了分析。WGSNP 分析显示存在三个不同的分支，巴西分离株属于两个分支 A 和 C。在所研究的菌株中，大多数基因和基因组区域存在差异。铁载体基因 iroB 和 iroC 仅存在于巴西分离株中，pegD 基因与菌毛粘附决定因素有关，在 A 群和 B 群的 124 株菌株中呈阳性，但在 C 群的所有菌株（n=71）中均为阴性。在巴西分离株中发现了 11 个质粒复制子，其中 9 个仅存在于其他国家的分离株中。在所有研究的菌株中均发现了编码外排泵的 mdsA 和 mdsB 抗菌药物耐药基因。巴西分离株还携带其他耐药基因，如 tet(A)（n=11）、tet(B)（n=4）和 tet(C)（n=4）、blaTEM-1（n=4）、catA1（n=1）、aadA1（n=1）和 sul1（n=1）。总之，巴西分离的都柏林沙门氏菌株存在一些在其他国家分离株中未发现的独特基因，并且被分配到两个不同的分支中，这些分支与人类和动物来源的菌株具有流行病学相关性。这一事实强调了在巴西流行了 30 多年的都柏林沙门氏菌的人畜共患潜力。
