School of Life, Health and Chemical Sciences, The Open University, Milton Keynes, UK.
Department of Experimental Oncology, European Institute of Oncology IRCCS, Milan, Italy.
Methods Mol Biol. 2022;2492:315-331. doi: 10.1007/978-1-0716-2289-6_19.
Adhesion between leukocytes and brain endothelial cells, which line cerebral blood vessels, is a key event in both physiological and pathological conditions such as neuroinflammatory diseases. Leukocyte recruitment from blood into tissues is described as a multistep process involving leukocyte rolling on endothelial cells, adhesion, crawling, and diapedesis under hemodynamic shear stress. In neuroinflammatory conditions, there is an increase in leukocyte adhesion to the brain endothelial cells, activated by proinflammatory molecules such as cytokines. Here, we describe an in vitro technique to study the interaction between human leukocytes with human brain endothelial cells under shear stress mimicking the blood flow in vivo, coupled to live-cell imaging.
白细胞与脑内皮细胞（衬附在脑血管上）之间的黏附是生理和病理条件下（如神经炎症性疾病）的一个关键事件。白细胞从血液向组织中的募集被描述为一个多步骤过程，包括白细胞在内皮细胞上的滚动、黏附、爬行和在血流切应力下的穿越。在神经炎症条件下，白细胞与脑内皮细胞的黏附增加，这是由细胞因子等促炎分子激活的。在这里，我们描述了一种在体外模拟体内血流剪切应力的条件下，研究人类白细胞与人类脑内皮细胞相互作用的技术，结合了活细胞成像。
