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中国白梨基因的综合分析：在叶片再生中的关键作用

Integrative Analysis of the Genes From Chinese White Pear (): A Critical Role in Leaf Regeneration.

作者信息

Wang Xinya, Manzoor Muhammad Aamir, Wang Mengna, Zhao Yu, Feng Xiaofeng, Alam Pravej, Chi Xujing, Cai Yongping

机构信息

School of Life Sciences, Anhui Agricultural University, Hefei, China.

Department of Biology, College of Science and Humanities, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia.

出版信息

Front Plant Sci. 2022 Jun 6;13:898786. doi: 10.3389/fpls.2022.898786. eCollection 2022.

DOI:10.3389/fpls.2022.898786
PMID:35734253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9208361/
Abstract

is a transcription regulator factor, which plays an important role in plant growth and development. Previous analyses found that several functions have been identified, such as axillary bud meristem formation, radial root elongation, gibberellin signaling, light signaling, and abiotic stress. The family has been comprehensively evaluated in several species. However, little finding is on the transcription factors (TFs) in Chinese white pear. In this study, 99 were systemically characterized and renamed 1 to 99 according to their chromosomal localizations. Phylogenetic analysis and structural features revealed that could be classified into eight subfamilies (LISCL, Ls, SHR, HAM, SCL, PAT, SCR, and DELLA). Further analysis of introns/exons and conserved motifs revealed that they are diverse and functionally differentiated in number and structure. Synteny analysis among , and showed that duplicated regions were more conserved. Dispersed duplication events are the most common mechanism and may play a crucial role in the expansion of the gene family. In addition, is-acting elements of the gene were found in promoter regions associated with hormone and environmental stress responses. Notably, the expression pattern detected by qRT-PCR indicated that genes were differentially expressed under gibberellin (GA), abscisic acid (ABA), and auxin (IAA) conditions, which are responsive to abiotic stress. and were highly expressed at different stages of hormone treatment and may play important role in leaf development. Therefore, we selected to clone and construct pCAMBIA1301- and transferred them into . Finally, we observed and compared the changes of overexpressed plants and wild-type plants during regeneration. This method was used to analyze their roles in leaf regeneration of Chinese white pear. In addition, we also constructed pCAMBIA1305-, and transferred them into onion cells to determine the subcellular localization. Subcellular localization experiments showed that and were localized in the nucleus. In summary, the results of this study indicate that and are mainly responsible for leaf regeneration of Chinese white pear, which plays a positive role in callus formation and provides rich resources for studying gene functions.

摘要

是一种转录调节因子，在植物生长发育中起重要作用。先前的分析发现已鉴定出几种功能，如腋芽分生组织形成、根径向伸长、赤霉素信号传导、光信号传导和非生物胁迫。该家族已在多个物种中得到全面评估。然而，关于中国白梨中的转录因子（TFs）的研究发现很少。在本研究中，对99个进行了系统表征，并根据它们在染色体上的定位重新命名为1至99。系统发育分析和结构特征表明可分为八个亚家族（LISCL、Ls、SHR、HAM、SCL、PAT、SCR和DELLA）。对内含子/外显子和保守基序的进一步分析表明，它们在数量和结构上是多样的且功能有差异。、和之间的共线性分析表明重复区域更保守。分散重复事件是最常见的机制，可能在基因家族的扩展中起关键作用。此外，在与激素和环境胁迫反应相关的启动子区域发现了基因的顺式作用元件。值得注意的是，通过qRT-PCR检测的表达模式表明，基因在赤霉素（GA）、脱落酸（ABA）和生长素（IAA）条件下差异表达，这些条件对非生物胁迫有响应。和在激素处理的不同阶段高度表达，可能在叶片发育中起重要作用。因此，我们选择克隆并构建pCAMBIA1301-，并将它们转入。最后，我们观察并比较了过表达植物和野生型植物在再生过程中的变化。该方法用于分析它们在中国白梨叶片再生中的作用。此外，我们还构建了pCAMBIA1305-，并将它们转入洋葱细胞以确定亚细胞定位。亚细胞定位实验表明和定位于细胞核。总之，本研究结果表明和主要负责中国白梨的叶片再生，在愈伤组织形成中起积极作用，并为研究基因功能提供了丰富的资源。

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